In vitro oxidation of LDL by ozone
| Autor: | Gerhard Cvirn, Gerd Kager, Günther Jürgens, Gerhard Ledinski, Erwin Tafeit, Gerd Hörl, Seth Hallström, Martin Koestenberger |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Apolipoprotein B biology Thiobarbituric acid Organic Chemistry NF-κB Cell Biology Oxidative phosphorylation Biochemistry Epitope Lipoproteins LDL chemistry.chemical_compound Kinetics Ozone chemistry Low-density lipoprotein biology.protein TBARS Humans lipids (amino acids peptides and proteins) Molecular Biology Oxidation-Reduction Lipoprotein |
| Zdroj: | Chemistry and physics of lipids. 183 |
| ISSN: | 1873-2941 |
| Popis: | Recent studies suggest that ozone is present in atherosclerotic lesions. Since these lesions are characterized by a dramatic accumulation of low-density lipoprotein (LDL), we aimed to investigate whether ozone is capable of oxidizing LDL, thereby rendering this lipoprotein atherogenic. Lipid hydroperoxide (LPO) concentrations and thiobarbituric acid reactive substances (TBARS) were measured to assess the oxidative status of the lipid part of LDL. Relative electrophoretic mobility (REM) and oxidation-specific immune epitopes were measured to assess the oxidative status of the protein part (apoB) of the LDL particle. Ozone turned out to be a potent oxidant of LDL. LPO concentrations, TBARS, REM, and oxidation-specific immune epitopes significantly increased upon ozonization. Our results suggest that ozonization of LDL may be a novel pathway which supports atherogenesis. Ozone is capable of oxidizing the lipid part of LDL, followed by immediate oxidation of the protein part of LDL, rendering the lipoprotein atherogenic. |
| Databáze: | OpenAIRE |
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