The Drosophila melanogaster transcriptome by paired-end RNA sequencing
| Autor: | Wei Li, Liguo Wang, Xia Wang, Richard A. Gibbs, David B. Emmert, Bryce Daines, Yi Han, Yumei Li, Hui Wang, William M. Gelbart, Rui Chen |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Resource Genetics Sequence Analysis RNA Gene Expression Profiling Molecular Sequence Data Alternative splicing Molecular Sequence Annotation Biology biology.organism_classification Transcriptome Alternative Splicing Exon Drosophila melanogaster Genes Y-Linked RNA splicing Melanogaster Animals Female FlyBase : A Database of Drosophila Genes & Genomes Gene Genetics (clinical) Oligonucleotide Array Sequence Analysis |
| Zdroj: | Genome Research. 21:315-324 |
| ISSN: | 1088-9051 |
| Popis: | RNA-seq was used to generate an extensive map of the Drosophila melanogaster transcriptome by broad sampling of 10 developmental stages. In total, 142.2 million uniquely mapped 64–100-bp paired-end reads were generated on the Illumina GA II yielding 356× sequencing coverage. More than 95% of FlyBase genes and 90% of splicing junctions were observed. Modifications to 30% of FlyBase gene models were made by extension of untranslated regions, inclusion of novel exons, and identification of novel splicing events. A total of 319 novel transcripts were identified, representing a 2% increase over the current annotation. Alternate splicing was observed in 31% of D. melanogaster genes, a 38% increase over previous estimations, but significantly less than that observed in higher organisms. Much of this splicing is subtle such as tandem alternate splice sites. |
| Databáze: | OpenAIRE |
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