Enterovirus 71 infection of human airway organoids reveals VP1-145 as a viral infectivity determinant
| Autor: | Dasja Pajkrt, Sylvie M. Koekkoek, Hans Clevers, Norman Sachs, Gerrit Koen, Katja C. Wolthers, Sabine M. G. van der Sanden |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research, AII - Infectious diseases, Medical Microbiology and Infection Prevention, ARD - Amsterdam Reproduction and Development, Paediatric Infectious Diseases / Rheumatology / Immunology |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Epidemiology viruses Immunology Amino Acid Motifs Virulence Enterovirus A Human/chemistry Biology Virus Replication Microbiology Virus Article 03 medical and health sciences Virology Drug Discovery Enterovirus 71 Organoid Enterovirus Infections Human/chemistry Humans Capsid Proteins/chemistry Amino Acid Sequence Infectivity Organoids/virology virus diseases General Medicine biology.organism_classification Human genetics 3. Good health Enterovirus A Human Organoids Kinetics 030104 developmental biology Infectious Diseases Parasitology Viral replication Capsid Proteins Enterovirus A Sequence Alignment Enterovirus Infections/virology |
| Zdroj: | Emerging Microbes & Infections Emerging Microbes & Infections, 7(1). Nature Publishing Group Emerging Microbes and Infections, 7(1). Nature Publishing Group Emerging microbes and infections, 7(1):00772. Nature Publishing Group |
| ISSN: | 2222-1751 |
| Popis: | Human enteroviruses frequently cause severe diseases in children. Human enteroviruses are transmitted via the fecal-oral route and respiratory droplets, and primary replication occurs in the gastro-intestinal and respiratory tracts; however, how enteroviruses infect these sites is largely unknown. Human intestinal organoids have recently proven to be valuable tools for studying enterovirus-host interactions in the intestinal tract. In this study, we demonstrated the susceptibility of a newly developed human airway organoid model for enterovirus 71 (EV71) infection. We showed for the first time in a human physiological model that EV71 replication kinetics are strain-dependent. A glutamine at position 145 of the VP1 capsid protein was identified as a key determinant of infectivity, and residues VP1-98K and VP1-104D were identified as potential infectivity markers. The results from this study provide new insights into EV71 infectivity in the human airway epithelia and demonstrate the value of organoid technology for virus research. |
| Databáze: | OpenAIRE |
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