Stability of the human faecal microbiome in a cohort of adult men
| Autor: | Paul Lochhead, Curtis Huttenhower, Andrew T. Chan, Jacques Izard, Galeb Abu-Ali, Himel Mallick, Raaj S. Mehta, Hamed Khalili, Kerry L. Ivey, Gordon T. Brown, Jason Lloyd-Price, Ayshwarya Subramanian, David A. Drew, Long H. Nguyen, Eric B. Rimm, Casey DuLong, Mingyang Song, Amit Joshi |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male Health Personnel Immunology Gene Expression Biology Applied Microbiology and Biotechnology Microbiology Article Cohort Studies 03 medical and health sciences Feces Genetics Diagnostic biomarker Humans Microbiome Prospective Studies Prospective cohort study Aged Bacteria Gene Expression Profiling Microbiota Gastrointestinal Microbiome High-Throughput Nucleotide Sequencing Cell Biology Middle Aged Gene expression profiling 030104 developmental biology Metagenomics Evolutionary biology Cohort Cohort study Follow-Up Studies |
| Popis: | Characterizing the stability of the gut microbiome is important to exploit it as a therapeutic target and diagnostic biomarker. We metagenomically and metatranscriptomically sequenced the faecal microbiomes of 308 participants in the Health Professionals Follow-Up Study. Participants provided four stool samples—one pair collected 24–72 h apart and a second pair ~6 months later. Within-person taxonomic and functional variation was consistently lower than between-person variation over time. In contrast, metatranscriptomic profiles were comparably variable within and between subjects due to higher within-subject longitudinal variation. Metagenomic instability accounted for ~74% of corresponding metatranscriptomic instability. The rest was probably attributable to sources such as regulation. Among the pathways that were differentially regulated, most were consistently over- or under-transcribed at each time point. Together, these results suggest that a single measurement of the faecal microbiome can provide long-term information regarding organismal composition and functional potential, but repeated or short-term measures may be necessary for dynamic features identified by metatranscriptomics. Metagenomic and metatranscriptomic analyses of stool samples from 308 individuals over time indicate that longitudinal sampling is important for detecting dynamic functional features of the gut microbiome. |
| Databáze: | OpenAIRE |
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