Cryopreservation of porcine embryos: state of the art
| Autor: | Michel Terqui, Françoise Berthelot, Françoise Martinat-Botté, Gábor Vajta |
|---|---|
| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
animal structures
Cryoprotectant [SDV]Life Sciences [q-bio] [INFO] Computer Science [cs] Biology Cryopreservation Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Glycerol [INFO]Computer Science [cs] Vitrification Survival rate ComputingMilieux_MISCELLANEOUS 030219 obstetrics & reproductive medicine General Veterinary business.industry 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences Straw 040201 dairy & animal science Biotechnology [SDV] Life Sciences [q-bio] chemistry embryonic structures Animal Science and Zoology business |
| Zdroj: | Berthelot, F, Martinat-Botte, F, Terqui, M & Vajta, G 2003, ' Cryopreservation of porcine embryos: state of the art ', Livestock Production Science, vol. 83, no. 1, pp. 73-83 . https://doi.org/10.1016/S0301-6226(03)00038-1 Livestock Production Science Livestock Production Science, Elsevier, 2003, 83, pp.73-83 Berthelot, F, Martinat-Botte, F, Vajta, G & Terqui, M 2001, Cryopreservation of porcine embryos: state of the art . in Proc. of the 52th Annual Meeting of the European Association of Animal Production . pp. 210, Budapest, Hungary, 18/12/2010 . |
| ISSN: | 0301-6226 |
| DOI: | 10.1016/S0301-6226(03)00038-1 |
| Popis: | Long term storage of porcine embryos would be worthwhile both for the preservation of genetic resources and for exportation of genotypes around the world. Three methods are available: freezing, classical vitrification and Open Pulled Straw vitrification (OPS). The efficiency of these methods were mainly evaluated in vitro by the development of morulae and blastocysts and in vivo by the birth rate after transfer into recipients. Freezing involved addition of glycerol as the cryoprotectant, the temperature is slowly decreased with, at least one step. Despite some development in vitro, the in vivo survival after transfer of embryos up to the peri-hatching stage is lower than 5%. Ice crystal formation was considered to be responsible for the main damages to embryos. Vitrification that allows direct transition between liquid and vitreous phase was the second method developed. When combined with pre and post-cryopreservation treatments of the embryos, the in vivo survival rate rises up to 18.5%. The OPS method is characterised by a very high speed of vitrification and without any treatment of embryos, the in vivo survival of embryos was close to 30%. In conclusion, methods are now available for cryopreservation of porcine embryos and further improvements are expected for a better efficiency and practicability. |
| Databáze: | OpenAIRE |
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