Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor and interferon
| Autor: | J S H Gaston, Markus Rihl, D T Y Yu, J G Kuipers, Elisabeth Märker-Hermann, Henning Zeidler, D.L. Baeten, Jane C. Goodall, Jieruo Gu |
|---|---|
| Přispěvatelé: | Clinical Immunology and Rheumatology |
| Rok vydání: | 2004 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Concise Report Transcription Genetic Receptors Antigen T-Cell alpha-beta medicine.medical_treatment T cell Immunology Cytokine Expression Profile CD8-Positive T-Lymphocytes Biology Arthritis Reactive General Biochemistry Genetics and Molecular Biology Interferon-gamma Rheumatology T-Lymphocyte Subsets Prohibitins Synovial Fluid medicine Humans Immunology and Allergy Interferon gamma Gamma delta T cell Oligonucleotide Array Sequence Analysis Tumor Necrosis Factor-alpha Receptors Antigen T-Cell gamma-delta T lymphocyte Middle Aged Molecular biology Clone Cells medicine.anatomical_structure Cytokine Cytokines Tumor necrosis factor alpha CD8 medicine.drug |
| Zdroj: | Annals of the rheumatic diseases, 63(12), 1673-1676. BMJ Publishing Group |
| ISSN: | 0003-4967 |
| DOI: | 10.1136/ard.2003.018192 |
| Popis: | OBJECTIVE: To compare the cytokine expression profile of three CD8+, three CD4+, and three gammadelta+ T cell clones all derived from the synovial fluids of three patients with reactive arthritis (ReA). METHODS: Complementary DNA based microarrays containing the specific sequence of 56 cytokine transcripts were used for screening. Selected genes were confirmed by reverse transcriptase-polymerase chain reaction assay. RESULTS: Microarray showed that transcripts encoding for interferon gamma and tumour necrosis factor alpha were expressed by all CD8+ and CD4+ T cell clones. However, gammadelta+ T cells predominantly expressed transforming growth factor beta2 and granulocyte monocyte-colony stimulating factor. CONCLUSION: T lymphocyte clones from the joint of patients with ReA exhibit differential cytokine expression profiles. CD8+ and CD4+ T cells demonstrate a Th1 mediated profile, whereas gammadelta+ T cells show a more heterogeneous and less proinflammatory Th3 driven pattern |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|