Evaluation of the use of serum lathosterol concentration to assess whole-body cholesterol synthesis in rabbits
| Autor: | H. A. Van Der Voort, H. J. M. Kempen, J. G. P. Van Der Palen, A. C. Beynen, H. de Vries, L.F.M. van Zutphen, G. W. Meijer |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Very low-density lipoprotein Lathosterol QD415-436 Biology Biochemistry chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Inbreeding Cholestyramine Lagomorpha Cholesterol Cell Biology biology.organism_classification Hydroxymethylglutaryl-CoA reductase Sterol chemistry Evaluation Studies as Topic Simvastatin lipids (amino acids peptides and proteins) Rabbits medicine.drug |
| Zdroj: | Journal of Lipid Research, Vol 33, Iss 2, Pp 281-286 (1992) |
| ISSN: | 0022-2275 |
| Popis: | Serum lathosterol concentration in rabbits was assessed as a possible indicator of whole-body cholesterol synthesis. In random-bred New Zealand White (NZW) rabbits fed a control diet or a diet containing either cholesterol, simvastatin, or cholestyramine, neither serum lathosterol concentration nor the serum lathosterol:total cholesterol ratio systematically corresponded with the anticipated rate of cholesterol synthesis. In control rabbits and those fed simvastatin or cholestyramine, whole-body cholesterol synthesis, which was calculated from the sterol balance, was correlated with serum lathosterol concentration when expressed relative to cholesterol in very low, intermediate, and low density lipoproteins (VLDL + IDL + LDL) (r = 0.61; n = 23; P = 0.002). The low correlation coefficient indicates that the predictive value of the lathosterol: (VLDL + IDL + LDL) cholesterol ratio is limited when applied to individual rabbits. Cholesterol and simvastatin feeding reduced the group mean serum lathosterol:(VLDL + IDL + LDL) cholesterol ratio, whereas cholestyramine in the diet raised the group mean ratio in the NZW rabbits. We conclude that the serum lathosterol:(VLDL + IDL + LDL) cholesterol ratio may be an indicator of group mean rates of whole-body cholesterol synthesis in rabbits but may not yield reliable information on individual rabbits. The lathosterol:(VLDL + IDL + LDL) cholesterol ratio predicted that in hyperresponsive inbred rabbits, showing an excessive hypercholesterolemia after cholesterol feeding, baseline whole-body cholesterol synthesis is lower than in hyporesponsive rabbits. Addition of cholesterol to the diet caused a reduction of predicted cholesterol synthesis in hypo- but not in hyper-responsive rabbits. |
| Databáze: | OpenAIRE |
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