Recent developments of magnetic beads for use in nucleic acid purification
| Autor: | Ian J. Bruce, Peter R. Levison, Dieter Schimkat, Prit Hathi, Stephen E. Badger, Jon W. Dennis, Martin J. Davies |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Male Lysis Oligonucleotides medicine.disease_cause Biochemistry Analytical Chemistry Sepharose Magnetics chemistry.chemical_compound Salmon Cricetinae Nucleic Acids Escherichia coli medicine Animals RNA Messenger Anion Exchange Resins pBluescript Chromatography Chemistry Organic Chemistry Nucleic acid methods General Medicine Chromatography Ion Exchange Spermatozoa Agarose Alkaline lysis DNA Plasmids |
| Zdroj: | Journal of Chromatography A. 816:107-111 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(98)00064-8 |
| Popis: | The performance of Magarose, an agarose-based bead containing a paramagnetic component has been evaluated. The anion exchanger DEAE-Magarose is effective at binding DNA from a crude cell lysate. The plasmid pBluescript was isolated from 1.5 ml Escherichia coli JM109 cell culture, following alkaline lysis yielding 8.2 micrograms high-quality DNA. Under similar binding conditions 21 micrograms of salmon sperm DNA bound to the ion exchangers. The affinity medium oligo-dT Magarose was demonstrated to bind 75 mumol of an oligo-dA probe/g of medium by hybridization. Under similar conditions mRNA could be isolated from a preparation of baby hamster cell total RNA. The magnetic susceptibility of Magarose is very high, facilitating the use of this separation technique for rapid batch chromatographic processes. |
| Databáze: | OpenAIRE |
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