Antibiotic resistances of intestinal lactobacilli isolated from wild boars
| Autor: | Corinna Kern, Katharina Bayer, Viviana Klose, Gertrude Wegl, Florian Goelß, Silvia Fibi |
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| Rok vydání: | 2014 |
| Předmět: |
Genotype
Tetracycline medicine.medical_treatment Sus scrofa Dalfopristin Erythromycin Microbial Sensitivity Tests Biology Polymerase Chain Reaction Microbiology Minimum inhibitory concentration RNA Ribosomal 16S medicine Animals General Veterinary Quinupristin Chloramphenicol food and beverages Drug Resistance Microbial Kanamycin General Medicine biochemical phenomena metabolism and nutrition Anti-Bacterial Agents Random Amplified Polymorphic DNA Technique Intestines Lactobacillus Streptomycin medicine.drug |
| Zdroj: | Veterinary Microbiology. 168:240-244 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2013.11.014 |
| Popis: | Acquired antibiotic resistances have been reported in lactobacilli of various animal and food sources, but there are no data from wild boar. The objective was a preliminary examination of the antibiotic resistance prevalence of intrinsically vancomycin-resistant lactobacilli isolated from wild boar intestines and analysis of the genetic determinants implicated. Out of three wild boars, 121 lactobacilli were recovered and grouped according to their whole cell protein patterns. Initial phenotypic screening revealed that all were susceptible to erythromycin (2 μg/ml), but 30 were resistant to tetracycline (32 μg/ml). Based on Randomly Amplified Polymorphic DNA-PCR clustering, 64 strains were selected as representative genotypes for identification and minimum inhibitory concentration (MIC) determination. Partial 16S rRNA gene sequencing identified four species: (i) L. mucosae (n=57), (ii) L. reuteri (n=47), (iii) L. fermentum (n=12), and (iv) L. murinus (n=5). Most heterofermentative strains displayed low MICs for ampicillin (AMP), chloramphenicol (CHL), streptomycin (STR), kanamycin (KAN), gentamicin (GEN), erythromycin (ERY), quinupristin/dalfopristin (Q/D), and clindamycin (CLI). Atypical MICs were found mainly in L. mucosae and L. reuteri for TET, KAN, STR, AMP and CHL, but except the TET MICs of L. mucosae mostly at low level. L. murinus strains revealed atypical MICs for aminoglycosides, and/or CHL, AMP, CLI. PCR screening detected tet(W) in 12 and tet(M) in one of heterofermentative strains, as well as the aph(3')-III kanamycin gene in L. murinus. This is the first report showing acquired antibiotic resistance determinants in intestinal lactobacilli of wild boar origin. |
| Databáze: | OpenAIRE |
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