Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick
| Autor: | Katherine A. Mirica, Rebecca Cademartiri, Sarah J. Vella, Andres W. Martinez, Scott T. Phillips, George M. Whitesides, Patrick Beattie, Anna Laromaine |
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| Rok vydání: | 2012 |
| Předmět: |
Paper
Fingerstick 02 engineering and technology 01 natural sciences Sensitivity and Specificity Article Analytical Chemistry Liver Function Tests Blood plasma Calibration Humans Sample preparation Aspartate Aminotransferases Chromatography Chemistry 010401 analytical chemistry Blood Proteins Equipment Design Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Alkaline Phosphatase Blood proteins 0104 chemical sciences 3. Good health Liver Alkaline phosphatase Liver function 0210 nano-technology Quantitative analysis (chemistry) Filtration |
| Zdroj: | Analytical chemistry. 84(6) |
| ISSN: | 1520-6882 |
| Popis: | This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and “spiked” standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings. |
| Databáze: | OpenAIRE |
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