Type II topoisomerase mutations in clinical isolates of Enterobacter cloacae and other enterobacterial species harbouring the qnrA gene
| Autor: | J.-D. Cavallo, Emmanuelle Cambau, Claude-James Soussy, Jérôme Robert, Isabelle Podglajen, Marie-Cécile Ploy, Vincent Cattoir, Richard Bonnet, C. Lascols, Cécile Bébéar |
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| Přispěvatelé: | Biologie moléculaire et cellulaire des microorganismes (EA3175), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Limoges (UNILIM) |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Sequence Analysis
DNA Nalidixic acid MESH: DNA Topoisomerases Type II Quinolones medicine.disease_cause DNA gyrase MESH: Enterobacter cloacae [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases heterocyclic compounds Pharmacology (medical) MESH: Phylogeny MESH: Enterobacteriaceae Infections Phylogeny Antibacterial agent Genetics MESH: Microbial Sensitivity Tests 0303 health sciences Mutation biology Enterobacteriaceae Infections General Medicine Infectious Diseases DNA Gyrase [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH: Genes Bacterial MESH: DNA Gyrase medicine.drug DNA Topoisomerase IV Microbiology (medical) MESH: Mutation Topoisomerase IV Enterobacter Microbial Sensitivity Tests Microbiology 03 medical and health sciences Drug Resistance Bacterial Enterobacter cloacae MESH: Drug Resistance Bacterial medicine Humans 030304 developmental biology MESH: Quinolones MESH: Humans 030306 microbiology Topoisomerase Sequence Analysis DNA MESH: DNA Topoisomerase IV biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology DNA Topoisomerases Type II Genes Bacterial biology.protein MESH: Enterobacter Type II topoisomerase |
| Zdroj: | International Journal of Antimicrobial Agents International Journal of Antimicrobial Agents, Elsevier, 2007, 29 (4), pp.402-409. ⟨10.1016/j.ijantimicag.2006.11.008⟩ |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2006.11.008 |
| Popis: | International audience; The qnr genes are transferable genes that confer low-level quinolone resistance by protection of topoisomerase. The occurrence of mutations in DNA gyrase (gyrA, gyrB) and topoisomerase IV (parC, parE) genes in strains harbouring qnr was investigated in 28 qnrA-positive clinical isolates, among which 7 strains also harboured qnrS. Topoisomerase mutations were found in 25 (89%) of the 28 strains, with at least two mutations (gyrA and parC) in 13 strains and one mutation in 12 strains. Isolates of the Enterobacter cloacae complex were compared with reference strains of the new Enterobacter species. gyrA mutations were found at position 83 (Ser or Thr for Ile, Tyr, Leu or Phe depending on the species), and new gyrB mutations were described (S463A, S464F). qnrA had an additive effect of a 10-fold increase in the minimum inhibitory concentration (MIC) whatever the number of topoisomerase mutations, and qnrS was additive to qnrA with a further 2- to 10-fold increase in the MIC. Comparison of MICs with susceptibility breakpoints showed that strains combining qnrA and topoisomerase mutations were resistant to fluoroquinolones, but the three strains lacking a topoisomerase mutation were susceptible using ciprofloxacin and levofloxacin but not using nalidixic acid or moxifloxacin testing. |
| Databáze: | OpenAIRE |
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