Role of calcifying nanoparticles in the development of testicular microlithiasis in vivo
| Autor: | Xiang Gao, Bo Song, Gensheng Lu, Qing-hua Zhang, Xia-cong Lin |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Pathology medicine.medical_specialty Urology Lumen (anatomy) 02 engineering and technology lcsh:RC870-923 Testicular Diseases Testicular microlithiasis Calculi Calcification Rats Sprague-Dawley 03 medical and health sciences Calcifying Nanoparticles Mice 0302 clinical medicine In vivo Testis medicine Escherichia coli Animals Humans Inflammation business.industry Calcinosis General Medicine Seminiferous Tubules 021001 nanoscience & nanotechnology medicine.disease lcsh:Diseases of the genitourinary system. Urology Sperm Rats Disease Models Animal Seminiferous tubule medicine.anatomical_structure Reproductive Medicine 030220 oncology & carcinogenesis Ultrastructure Microscopy Electron Scanning 0210 nano-technology business Research Article |
| Zdroj: | BMC Urology BMC Urology, Vol 17, Iss 1, Pp 1-6 (2017) |
| ISSN: | 1471-2490 |
| Popis: | Background Calcifying nanoparticles (NPs) have been proven to be associated with a variety of pathological calcification and previously detected in semen samples from patients with testicular microlithiasis (TM). The present study was designed to test the hypothesis if human-derived NPs could invade the seminiferous tubules and induce TM phenotype. Methods The animals were divided into three groups. Normal saline (0.2 mL) was injected into the proximal right ductus deferens in group A as a control group. The experimental groups, B and C received Escherichia coli (106 cfu/mL, 0.2 mL) and human-derived NPs suspension (0.2 mL), respectively. Rats were euthanized in 2 batches at 2 and 4 weeks. Testicular pathology, ultrastructure and inflammatory mediators were assessed. Results Chronic inflammatory changes were observed at 2 weeks in both groups B and C. Moreover, the innermost layer of sperm cells were structurally impaired and a zone of concentrically layered collagen fibers around the human NPs body was formed in the lumen of the seminiferous tubule in group C only, in which TM phenotype of remarkable calcification surrounded by cellular debris within the seminiferous tubules was built at 4 weeks. Conclusions The results obtained from our study suggested a potential pathogenic effect of NPs in the development of calcification within the seminiferous tubules, which should be addressed in the future studies. |
| Databáze: | OpenAIRE |
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