| Autor: |
Austin L. Shug, Dean Fraser, Henry R. Mahler |
| Rok vydání: |
1960 |
| Předmět: |
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| Zdroj: |
Biochimica et Biophysica Acta. 42:255-271 |
| ISSN: |
0006-3002 |
| DOI: |
10.1016/0006-3002(60)90789-7 |
| Popis: |
A variety of inhibitors has been shown to have pronounced effects on the replication of T2 bacteriophage in protoplasts of E. coli B. Ribonuclease applied in 5 or 10 min pulses exerts a maximum effect (40% reduction of burst size) when applied between 5 and 10 min after infection. p -chloromercury benzoate shows a sharp maximum of effectiveness and inhibits the burst completely when added 15 to 20 min after infection. Of a variety of purine and pyrimidine antagonists, 5-fluorouridine and 2′-deoxy-5-fluorouridine showed the most striking effects. The latter compound appears to have a specific inhibitory action on DNA synthesis and the inhibition (some 98%) is essentially completely reversed by thymidine. The action of 5-fluorouridine appears to be considerably more complex. In part its effect is also directly on DNA synthesis and is reversible by thymidine. During the first 10 min of infection, however, this inhibitor, especially when tested in the presence of thymidine, appears to exert its primary effect of RNA synthesis with resulting effects on the synthesis of “early protein” and hence DNA. These effects can be overcome by uridine. A combination of thymidine and uridine completely abolishes the inhibition by 5-fluorouridine even at quite high concentrations. These conclusions have been reached on the basis of measurements of phage yield and also of incorporation of 35 SO 4 and 32 PO 4 into the appropriate macromolecules. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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