Review of CRISPR/Cas9 sgRNA Design Tools
| Autor: | Xiangke Liao, Minxia Cheng, Jiaming Xu, Shaoliang Peng, Yingbo Cui |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene Editing Computer science Health Informatics Computational biology Genome General Biochemistry Genetics and Molecular Biology Computer Science Applications 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Genome editing Genome research Genetic Techniques Computational Science and Engineering CRISPR Animals Humans CRISPR-Cas Systems 030217 neurology & neurosurgery Subgenomic mRNA RNA Guide Kinetoplastida |
| Zdroj: | Interdisciplinary sciences, computational life sciences. 10(2) |
| ISSN: | 1867-1462 |
| Popis: | The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a powerful gene editing tool and got a broad application in genome research field due to its ease of use and cost-effectiveness. The performance of CRISPR/Cas relies on well-designed single-guide RNA (sgRNA), so a lot of bioinformatic tools have been developed to assist the design of highly active and specific sgRNA. These tools vary in design specifications, parameters, genomes and so on. To help researchers to choose their proper tools, we reviewed various sgRNA design tools, mainly focusing on their on-target efficiency prediction model and off-target detection algorithm. |
| Databáze: | OpenAIRE |
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