Occurrence, phylogeny and evolution of ribulose-1,5-bisphosphate carboxylase/oxygenase genes in obligately chemolithoautotrophic sulfur-oxidizing bacteria of the genera Thiomicrospira and Thioalkalimicrobium
| Autor: | Elizaveta M. Spiridonova, Ivan A. Berg, Dimitry Y. Sorokin, Boris B. Kuznetsov, Tatjana P. Tourova |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Ribulose 1
5-bisphosphate Base Sequence biology Phylogenetic tree Ribulose-Bisphosphate Carboxylase Molecular Sequence Data RuBisCO 16S ribosomal RNA biology.organism_classification Polymerase Chain Reaction Microbiology Evolution Molecular Monophyly chemistry.chemical_compound Biochemistry chemistry Phylogenetics Gammaproteobacteria biology.protein Piscirickettsiaceae Codon Phylogeny Thiomicrospira crunogena |
| Zdroj: | Microbiology. 152:2159-2169 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.28699-0 |
| Popis: | The occurrence of the different genes encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the key enzyme of the Calvin–Benson–Bassham cycle of autotrophic CO2fixation, was investigated in the members of the genusThiomicrospiraand the relative genusThioalkalimicrobium, all obligately chemolithoautotrophic sulfur-oxidizingGammaproteobacteria. ThecbbLgene encoding the ‘green-like’ form I RubisCO large subunit was found in all analysed species, while thecbbMgene encoding form II RubisCO was present only inThiomicrospiraspecies. Furthermore, species belonging to theThiomicrospira crunogena16S rRNA-based phylogenetic cluster also possessed two genes of green-like form I RubisCO,cbbL-1andcbbL-2. Both 16S-rRNA- andcbbL-based phylogenies of theThiomicrospira–Thioalkalimicrobium–Hydrogenovibriogroup were congruent, thus supporting its monophyletic origin. On the other hand, it also supports the necessity for taxonomy reorganization of this group into a new family with four genera. |
| Databáze: | OpenAIRE |
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