Expression of interleukin-15 and its receptor on the surface of stimulated human umbilical vein endothelial cells
| Autor: | Xiuping Liu, Deguang Yang, Changyun Xiong, Xiaozhou Zhang, Weina Zhang, Yumei Zuo |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_treatment
Biomedical Engineering Biology Biochemistry Umbilical vein Biomaterials Interferon-gamma Genetics medicine Human Umbilical Vein Endothelial Cells Humans Interferon gamma Northern blot RNA Messenger Cells Cultured Earth-Surface Processes Interleukin-15 Receptors Interleukin-15 Tumor Necrosis Factor-alpha Molecular biology Cell biology Up-Regulation Blot Interleukin-15 receptor Cytokine Interleukin 15 Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 29(5) |
| ISSN: | 1672-0733 |
| Popis: | Human interleukin-15 (hIL-15) is an important cytokine to activate endothelial cells and can be regulated by many other cytokines. The aim of this study is to examine the ability of interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) to induce the production of human interleukin-15 (hIL-15) and IL-15 receptor (IL-15Ralpha) by human umbilical vein endothelial cells (HUVECs). The data are summarized as follows: 1. Northern blot revealed that IL-15 mRNA was up-regulated by IFN-gamma and TNF-alpha. 2. Intracellular IL-15 protein was visualized by fluorescence microscopy, whereas the expression of IL-15 on the surface of HUVECs was detected by fluorescence activated cell sorting (FACS), and no detectable IL-15 in the medium was verified by ELISA. 3. IL-15Ralpha was detected on the surface of HUVECs by FACS after IFN-gamma and TNF-alpha stimulation, whereas Western blotting revealed that the elevated expression on surface IL-15Ralpha was not due to the increased protein expression. The conclusion demonstrated from our results is that IFN-gamma and TNF-alpha play an important role in regulating the expression of IL-15 and IL-15Ralpha on the surface of HUVECs. |
| Databáze: | OpenAIRE |
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