Matrix metalloproteinase 9-activatable peptide-conjugated hydrogel-based fluorogenic intraocular-lens sensor
| Autor: | Chae Eun Moon, Byunghoon Kang, Yong Woo Ji, Hyo Lee, Myeong Hoon Kim, Woo Seok Jinn, Hyung Keun Lee, Seungjoo Haam, Moo Kwang Shin, Jisun Ki, Byunggeol Mun |
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| Rok vydání: | 2019 |
| Předmět: |
genetic structures
medicine.medical_treatment Biomedical Engineering Biophysics Intraocular lens Peptide 02 engineering and technology Biosensing Techniques Conjugated system 01 natural sciences Cell Line Aqueous Humor In vivo Central Nervous System Diseases Electrochemistry medicine Animals Humans Fluorescent Dyes chemistry.chemical_classification Lenses Intraocular 010401 analytical chemistry Matrix metalloproteinase 9 Hydrogels General Medicine 021001 nanoscience & nanotechnology eye diseases 0104 chemical sciences chemistry Matrix Metalloproteinase 9 Intraocular fluid Self-healing hydrogels Eye disorder sense organs Rabbits 0210 nano-technology Peptides Biomarkers Biotechnology Biomedical engineering |
| Zdroj: | Biosensorsbioelectronics. 162 |
| ISSN: | 1873-4235 |
| Popis: | The eye is an extension of the central nervous system (CNS) and contains aqueous humor (AH), which is a fluid rich in biomolecules secreted from intraocular tissues; thus, this organ allows for non-invasive visualization of early changes in CNS disorders. There is a growing interest in developing implantable devices, such as intraocular-lens (IOL), for specific medical uses, including intraocular monitoring. We describe a novel IOL-sensing system for detecting AH biomarkers via biocompatible enzyme-activatable fluorogenic hydrogel sensors. Matrix-metalloproteinase-9, a biomarker of degenerative CNS and eye disorders, was selected as a target. A peptide-probe-incorporated fluorogenic IOL (FIOL) was developed using diacrylamide-group-modified poly(ethyleneglycol) (PEGDAAm) biocompatible hydrogels, adjusting the hydrogel mesh size to allow selective penetration of the target while blocking non-targets, using label-free detection with semi-permanently implantable sensors, and demonstrating the clinical feasibility of FIOL through in vivo testing. This novel FIOL-based sensing system represents a promising approach for liquid biopsy of intraocular fluids. |
| Databáze: | OpenAIRE |
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