Macrophage permissiveness for Legionella pneumophila growth modulated by iron
| Autor: | Thomas W. Klein, Cathy Newton, Raymond Widen, Yoshimasa Yamamoto, S J Gebran, Herman Friedman |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Permissiveness
Mice Inbred A Ratón Iron Immunology Transferrin receptor Deferoxamine In Vitro Techniques Biology Microbiology Legionella pneumophila Mice Receptors Transferrin medicine Animals Macrophage chemistry.chemical_classification Transferrin biology.organism_classification Infectious Diseases chemistry Thioglycolates Macrophages Peritoneal bacteria Female Parasitology Apoproteins Intracellular Research Article medicine.drug |
| Zdroj: | Infection and Immunity. 62:564-568 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | We have investigated the modulation of iron in two populations of macrophages which differ in susceptibility to Legionella pneumophila intracellular proliferation. Previously, we reported that thioglycolate-elicited peritoneal macrophages obtained from the inbred A/J mouse strain readily support the intracellular growth of L. pneumophila, while resident macrophages from the same strain do not. In this study, we show that A/J elicited macrophages exhibit markedly higher expression of transferrin receptor and intracellular iron content than A/J resident macrophages. Furthermore, apotransferrin and desferrioxamine inhibited the intracellular proliferation of L. pneumophila in elicited macrophages, and this suppression was reversed by the additions of Fe-transferrin or ferric nitrilotriacetate. Fe-transferrin and ferric nitrilotriacetate did not further increase the intracellular proliferation of L. pneumophila in thioglycolate-elicited macrophages. However, ferric citrate and ferric nitrilotriacetate stimulated in a dose-dependent manner the growth of L. pneumophila in resident macrophages. Furthermore, equimolar concentrations of desferrioxamine reversed the stimulatory effect of iron in these resident cells. These data provide evidence supporting the hypothesis that differences in susceptibility to L. pneumophila growth between permissive elicited macrophages and nonpermissive resident macrophages from the A/J mouse strain are due to intracellular availability of iron. |
| Databáze: | OpenAIRE |
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