Optimal Concentration of Organic Solvents to be Used in the Broth Microdilution Method to Determine the Antimicrobial Activity of Natural Products Against Paenibacillus Larvae
| Autor: | Noelia Melina Cugnata, Maria Celeste Pellegrini, Elisa Guaspari, S. R. Fuselli, Rosa M. Alonso-Salces |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
natural product American foulbrood NATURAL PRODUCT Otras Ciencias Biológicas PAENIBACILLUS LARVAE Plant Science broth microdilution Biology Microbiology Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences Minimum inhibitory concentration paenibacillus larvae Food science purl.org/becyt/ford/1.6 [https] Paenibacillus larvae ANTIMICROBIAL ACTIVITY american foulbrood antimicrobial activity Organic solvent Broth microdilution AMERICAN FOULBROOD Antimicrobial 030104 developmental biology QL1-991 Insect Science organic solvent BROTH MICRODILUTION ORGANIC SOLVENT Zoology CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Journal of Apicultural Science, Vol 61, Iss 1, Pp 37-53 (2017) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2299-4831 |
| Popis: | American Foulbrood (AFB) is a bacterial disease, caused by Paenibacillus larvae, that affects honeybees (Apis mellifera). Alternative strategies to control AFB are based on the treatment of the beehives with antimicrobial natural substances such as extracts, essential oils and/or pure compounds from plants, honey by-products, bacteria and moulds. The broth microdilution method is currently one of the most widely used methods to determine the minimum inhibitory concentration (MIC) of a substance. In this regard, the fact that most natural products, due to their lipophilic nature, must be dissolved in organic solvents or their aqueous mixtures is an issue of major concern because the organic solvent becomes part of the dilution in the incubation medium, and therefore, can interfere with bacterial viability depending on its nature and concentration. A systematic study was carried out to determine by the broth microdilution method the MIC and the maximum non inhibitory concentration (MNIC) against P. larvae of the most common organic solvents used to extract or dissolve natural products, i.e. ethanol, methanol, acetonitrile, n-butanol, dimethylsulfoxide, and acidified hydromethanolic solutions. From the MIC and MNIC for each organic solvent, recommended maximum concentrations in contact with P. larvae were established: DMSO 5% (v/v), acetonitrile 7.5% (v/v), ethanol 7.5% (v/v), methanol 12% (v/v), n-butanol 1% (v/v), and methanol-water-acetic acid (1.25:98.71:0.04, v/v/v). Fil: Cugnata, Noelia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina Fil: Guaspari, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina Fil: Pellegrini, María Celeste. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina Fil: Fuselli, Sandra Rosa. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina Fil: Alonso Salces, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina |
| Databáze: | OpenAIRE |
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