A gene-specific promoter element is required for optimal expression of the histone H1 gene in S-phase
| Autor: | Stephen Dalton, J. R. E. Wells |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Biology Alpharetrovirus General Biochemistry Genetics and Molecular Biology Cell Line Histones Histone H1 Transcription (biology) Gene expression Transcriptional regulation Animals Promoter Regions Genetic Interphase Molecular Biology Gene Base Sequence General Immunology and Microbiology General Neuroscience Genes Homeobox Transfection Molecular biology Histone Genes Cell culture biology.protein Chromosome Deletion Chickens Research Article |
| Zdroj: | The EMBO Journal. 7:49-56 |
| ISSN: | 0261-4189 |
| Popis: | An H1 gene-specific element (H1-box, 5'-AAACACA-3') modulates S-phase expression of the gene in vivo as judged by analysis of transcripts from histone genes transfected into HeLa cells. Deletion or base-substitution of the element causes a 15- to 30-fold decrease in steady-state H1 mRNA levels in randomly growing cells and eliminates cell cycle control of transcription in synchronized cells. Mutations within the H1-specific element which abolish S-phase control of transcription also eliminate binding of a sequence-specific nuclear factor capable of binding specifically to this region in vitro. Transfection of multiple copies of H1-box elements into cells drastically decreases H1 mRNA levels, mimicking the effect observed when the motif is rendered non-functional by deletion or substitution mutagenesis. In contrast, introduction of mutated H1 elements into cells has no detectable effect. Together, these results imply that an interaction between the H1-box and a sequence-specific trans-acting factor modulates transcriptional control of H1 genes in vivo. |
| Databáze: | OpenAIRE |
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