Ultrastructural imaging of Candida albicans adhesion to rat genital epithelium through scanning and transmission electron microscopy
| Autor: | Lucélia Donatti, Edilson Damke, Márcia Regina Batista, Marcia Edilaine Lopes Consolaro, Marcia Aparecida Carrara, Mary Mayumi Taguti Irie, Luciene Setsuko Akimoto Gunther, Terezinha Inez Estivalet Svidzinski, Agenor Storti-Filho, Eliana Valéria Patussi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
biology
Hypha Chemistry Vacuole Genitalia Female biology.organism_classification Epithelium Corpus albicans Microbiology Rats medicine.anatomical_structure Microscopy Electron Transmission In vivo Candida albicans medicine Ultrastructure Cell Adhesion Microscopy Electron Scanning Animals Female Rats Wistar Instrumentation Endocervix |
| Zdroj: | Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. 16(3) |
| ISSN: | 1435-8115 |
| Popis: | The adhesion ofCandida albicansto the genital epithelium has not been fully investigatedin vivo. The objective of this study was to evaluate the ultrastructural aspects ofC. albicansadhesion in the lower genital system of female Wistar rats through scanning and transmission electron microscopy. The genital infection persisted until the end of the experiment, and all rats showed the same adhesion aspects. Various associated yeast/hyphae were observed in the lumen and adhered both at the vaginal and endocervical levels where the fungal filamentation process occurred. In the vaginal epithelium, closely adhered yeasts were observed as stretched strands bridging between yeasts and the epithelium surface. Different stages of the adhesion, where yeasts internalized into the epithelial cell inside a cytoplasmic vacuole, resembling endocytosis, and a wide fibrillar-floccular, glycocalyx-like layer on the yeasts were observed. On the endocervix, the adhesion occurred between the cilia. In the uterine body, only a yeast-like form was observed with superficial contact. This study reached the initial goal of demonstrating an experimental model forin vivostudies. Continuation of this line of research is important for studies of vulvovaginal candidiasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|