Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM + FLIM)
| Autor: | David S. Corcoran, Edwin A. Garcia Castano, Paul M. W. French, Frederik Görlitz, Mark A. A. Neil, Birgit Leitinger, Christopher Dunsby |
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| Přispěvatelé: | Medical Research Council, Biotechnology and Biological Sciences Research Council |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Diffraction lcsh:Applied optics. Photonics Fluorescence-lifetime imaging microscopy Microscope Nanostructure Materials science structured illumination microscopy fluorescence lifetime microscopy Förster energy transfer collagen receptor DNA 01 natural sciences law.invention Quantitative Biology::Cell Behavior 010309 optics 03 medical and health sciences Optics law Optical mapping 0103 physical sciences Radiology Nuclear Medicine and imaging Instrumentation DDR1 business.industry lcsh:TA1501-1820 Atomic and Molecular Physics and Optics 030104 developmental biology Förster resonance energy transfer Optoelectronics business Discoidin domain |
| Zdroj: | Photonics, Vol 4, Iss 3, p 40 (2017) Photonics; Volume 4; Issue 3; Pages: 40 |
| ISSN: | 2304-6732 |
| Popis: | We present a new microscope integrating super-resolved imaging using structured illumination microscopy (SIM) with wide-field optically sectioned fluorescence lifetime imaging (FLIM) to provide optical mapping of molecular function and its correlation with biological nanostructure below the conventional diffraction limit. We illustrate this SIM + FLIM capability to map FRET readouts applied to the aggregation of discoidin domain receptor 1 (DDR1) in Cos 7 cells following ligand stimulation and to the compaction of DNA during the cell cycle. |
| Databáze: | OpenAIRE |
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