Gossypol inhibition of mitosis, cyclin D1 and Rb protein in human mammary cancer cells and cyclin-D1 transfected human fibrosarcoma cells
| Autor: | M Sonenberg, D Hochhauser, D Jeoung, M Ligueros, M M Reidenberg, B Tang |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Fibrosarcoma Cyclin D Cyclin B Mitosis Breast Neoplasms Transfection Retinoblastoma Protein chemistry.chemical_compound Cyclin D1 Cyclins Tumor Cells Cultured Humans Cyclin Oncogene Proteins biology Cell Cycle Gossypol Retinoblastoma protein Cell cycle Oncology chemistry Cancer cell biology.protein Cancer research Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1997.330 |
| Popis: | The antiproliferative effects of gossypol on human MCF-7 mammary cancer cells and cyclin D1-transfected HT-1060 human fibrosarcoma cells were investigated by cell cycle analysis and effects on the cell cycle regulatory proteins Rb and cyclin D1. Flow cytometry of MCF-7 cells at 24 h indicated that 10 microM gossypol inhibited DNA synthesis by producing a G1/S block. Western blot analysis using anti-human Rb antibodies and anti-human cyclin D1 antibodies in MCF-7 cells and high- and low-expression cyclin D1-transfected fibrosarcoma cells indicated that, after 6 h exposure, gossypol decreased the expression levels of these proteins in a dose-dependent manner. Gossypol also decreased the ratio of phosphorylated to unphosphorylated Rb protein in human mammary cancer and fibrosarcoma cell lines. Gossypol (10 microM) treated also decreased cyclin D1-associated kinase activity on histone H1 used as a substrate in MCF-7 cells. These results suggest that gossypol might suppress growth by modulating the expression of cell cycle regulatory proteins Rb and cyclin D1 and the phosphorylation of Rb protein. Images Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 |
| Databáze: | OpenAIRE |
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