A Glycine-Rich Domain of hnRNP H/F Promotes Nucleocytoplasmic Shuttling and Nuclear Import through an Interaction with Transportin 1
| Autor: | Courtney Van Dusen, Lisa M. McNally, Lily Yee, Mark T. McNally |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Heterogeneous nuclear ribonucleoprotein
viruses Recombinant Fusion Proteins Molecular Sequence Data Nuclear Localization Signals Active Transport Cell Nucleus Glycine Biology environment and public health medicine Humans Amino Acid Sequence Molecular Biology Cell Nucleus Heterogeneous-Nuclear Ribonucleoprotein Group F-H Alternative splicing Intron Articles Cell Biology beta Karyopherins Molecular biology Protein Structure Tertiary Cell biology Alternative Splicing Cell nucleus medicine.anatomical_structure Mutation Transportin 1 RNA splicing health occupations Nuclear transport Protein Processing Post-Translational Sequence Alignment Nuclear localization sequence HeLa Cells |
| Zdroj: | Molecular and Cellular Biology. 30:2552-2562 |
| ISSN: | 1098-5549 |
| Popis: | Heterogeneous nuclear ribonucleoprotein (hnRNP) H and F are members of a closely related subfamily of hnRNP proteins that are implicated in many aspects of RNA processing. hnRNP H and F are alternative splicing factors for numerous U2- and U12-dependent introns. The proteins have three RNA binding domains and two glycine-rich domains and localize to both the nucleus and cytoplasm, but little is known about which domains govern subcellular localization or splicing activity. We show here that the central glycine-tyrosine-arginine-rich (GYR) domain is responsible for nuclear localization, and a nonclassical nuclear localization signal (NLS) was mapped to a short, highly conserved sequence whose activity was compromised by point mutations. Glutathione S-transferase (GST) pulldown assays demonstrated that the hnRNP H NLS interacts with the import receptor transportin 1. Finally, we show that hnRNP H/F are transcription-dependent shuttling proteins. Collectively, the results suggest that hnRNP H and F are GYR domain-dependent shuttling proteins whose posttranslational modifications may alter nuclear localization and hence function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|