Study of a 13-weeks, Repeated, Intramuscular Dose, Toxicity Test of Sweet Bee Venom in Sprague-Dawley Rats
| Autor: | Hyun-Min Kang, Chung-San Lim, Kwang-Ho Lee, Ki-Rok Kwon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis Dose medicine.medical_treatment pharmacopuncture H&E stain Physiology lcsh:Medicine Urine Fibrosis medicine Saline lcsh:Miscellaneous systems and treatments sweet bee venom Pharmacology intramuscular business.industry Fatty liver lcsh:R lcsh:RM1-950 medicine.disease lcsh:RZ409.7-999 rats lcsh:Therapeutics. Pharmacology Complementary and alternative medicine melittin Toxicity Original Article medicine.symptom business 13 weeks repeated dose toxicity |
| Zdroj: | Journal of Pharmacopuncture Journal of Pharmacopuncture, Vol 17, Iss 2, Pp 73-79 (2014) |
| ISSN: | 2234-6856 2093-6966 |
| Popis: | Objectives: This study was performed to analyze a 13week repeated dose toxicity test of Sweet Bee Venom (SBV) extracted from bee venom and administered in Sprague-Dawley (SD) rats. Methods: Male and female 5-week-old SD rats were treated once daily with SBV (high-dosage group: 0.28 mg/kg; medium-dosage group: 0.14 mg/kg; or low-dos age group: 0.07 mg/kg) for 13 weeks. Normal saline was administered to the control group in a similar manner (0.2 mL/kg). We conducted clinical observations, body weight measurements, ophthalmic examinations, urinalyses, hematology and biochemistry tests, and histological observations using hematoxylin and eosin (H&E) staining to identify any abnormalities caused by the SBV treatment. Results: During this study, no mortality was observed in any of the experimental groups. Hyperemia and a movement disorder were observed around the area of in all groups that received SBV treatment, with a higher occurrence in rats treated with a higher dosage. Male rats receiving in the high-dosage group showed a significant decrease in weight during the treatment period. Compared to the control group, no significant changes in the ophthalmic parameters, the urine analyses, the complete blood cell count (CBC), and the biochemistry in the groups treated with SBV. Compared to the control group, some changes in organ weights were observed in the medium-and the high-dosage groups, but the low-dosage group showed no significant changes. Histological examination of thigh muscle indicated cell infiltration, inflammation, degenera tion, and necrosis of muscle fiber, as well as fibrosis, in both the medium- and the high-dosage groups. Fatty liver change was observed in the periportal area of rats receiving medium and high dosages of SBV. No other organ abnormalities were observed. Conclusion: Our findings suggest that the No Ob served Adverse Effect Level (NOAEL) of SBV is approx imately 0.07 mg/kg in male and female SD rats. |
| Databáze: | OpenAIRE |
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