Age-related changes of the metabolic profile of rat cerebellar cortex: enzyme histochemical study
| Autor: | Francesco Amenta, Elena Bronzetti, Laura Felici, Fabio Ferrante |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Senescence
medicine.medical_specialty Cerebellum Aging Central nervous system Granular layer Reductase Biology Glucosephosphate Dehydrogenase Cerebellar Cortex Internal medicine medicine Neuropil Animals L-Lactate Dehydrogenase Histocytochemistry NADH Dehydrogenase Rats Inbred Strains Rats Succinate Dehydrogenase medicine.anatomical_structure Endocrinology Biochemistry Ageing Cerebellar cortex Developmental Biology |
| Zdroj: | Mechanisms of ageing and development. 44(3) |
| ISSN: | 0047-6374 |
| Popis: | The influence of aging on the metabolic profile of cerebellar cortex was studied in young (3-month-old), adult (12-month-old) and aged (26-month-old) male Sprague—Dawley rats using enzyme histochemical techniques. The following enzymatic activities related to energy transduction were examined: lactate-(LDH) and succinate-(SDH) dehydrogenases; NADH 2 -tetrazolium reductase (NADHD) and alpha-glycerophosphate-dehydrogenase (GPDH). The intensity of enzymatic staining within the neuropil of molecular and granular layers as well as within the cytoplasm of Purkinje neurons of young, adult and aged animals was assessed microphotometrically. In the molecular layer LSH, SDH and NADHD levels were reduced in old rats; GPDH was decreased both in adult and old animals. In Purkinje neurons no age-related changes of the enzymatic activities under study were observed. In the granular layer LDH and GPDH showed an age-dependent loss; SDH and NADHD were unchanged. The possibility that age-related changes of the enzymatic activities under study may be due to impaired energy production mechanisms and/or represent the consequence of reduced energetic needs resulting from the documented age-dependent loss of synapses in the molecular or in the granular layers of cerebellar cortex is discussed. |
| Databáze: | OpenAIRE |
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