Binding of 125I-epidermal growth factor in human uterus
| Autor: | Ch.V. Rao, N. Chegini, Najib G. Wakim, Joseph S. Sanfilippo |
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| Rok vydání: | 1986 |
| Předmět: |
Cell type
medicine.medical_specialty Histology Stromal cell Uterus Biology Endometrium Epithelium Pathology and Forensic Medicine Epidermal growth factor Internal medicine medicine Humans Endothelium Binding site Menstrual Cycle Epidermal Growth Factor urogenital system Myometrium Muscle Smooth Cell Biology Cell biology ErbB Receptors Arterioles Endocrinology medicine.anatomical_structure In utero Autoradiography Female hormones hormone substitutes and hormone antagonists |
| Zdroj: | Cell And Tissue Research. 246:543-548 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/bf00215194 |
| Popis: | Quantitative light-microscopic autoradiography was used on five human uteri at two different phases of the menstrual cycle to ascertain the cell types with binding sites for epidermal growth factor (EGF). The results revealed that stromal cells, glandular epithelium of endometrium, elongated and circular muscle cells of myometrium, smooth muscle and endothelial cells of arterioles in the basal endometrium and myometrium contained numerous silver grains following incubation with 125I-EGF. Coincubation with 100-fold excess unlabeled EGF resulted in a complete disappearance of silver grains from all cell types. Quantitative grain analysis indicated that stromal cells contained the highest number of EGF-binding sites (P less than 0.05) with no significant differences among the others (P greater than 0.05). There was no cyclic variation of EGF-binding to any of the uterine cell types. The present data demonstrate that all the cell types of human uterus, including arterioles, contain EGF-binding sites. This suggests that all the cells in human uterus subserving different functions are targets of EGF action. |
| Databáze: | OpenAIRE |
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