Photostable methylene blue‐loaded silica particles used as label for immunosorbent assay of Salmonella Typhimurium
| Autor: | Mithran Somasundrum, Werasak Surareungchai, Patsamon Rijiravanich, Nopchulee Cheeveewattanagul, Tran Thanh Tien |
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| Rok vydání: | 2019 |
| Předmět: |
Salmonella typhimurium
0106 biological sciences Analyte Salmonella Surface Properties Biomedical Engineering Bioengineering medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Absorbance 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Drug Discovery medicine Particle Size 030304 developmental biology Immunoassay Detection limit 0303 health sciences Chromatography Bioconjugation medicine.diagnostic_test biology Chemistry Process Chemistry and Technology General Medicine Photochemical Processes Silicon Dioxide Methylene Blue Polyclonal antibodies biology.protein Molecular Medicine Methylene blue Biotechnology |
| Zdroj: | Biotechnology and Applied Biochemistry. 66:842-849 |
| ISSN: | 1470-8744 0885-4513 |
| Popis: | Salmonella Typhimurium is a major cause of food poisoning. To solve the limitations of the routine enzyme linked immunosorbent assay such as laborious assay procedure, lack of long-term enzyme stability, and insufficient sensitivity, we provided a non-enzymatic colorimetric immunosorbent assay platform to overcome these problems. The highly photostable redox dye particles was constructed by silica particles (diameter = 598 ± 14.4 nm) loaded with methylene blue (Si-MB) and applied to be a label for immunoassay of S. Typhimurium. The sandwich assay format involved incubation of an analyte in a microplate wells modified with monoclonal anti-Salmonella, followed by exposure to a polyclonal anti-Salmonella/Si-MB bioconjugate and then measurement of absorbance at 598 nm. The platform had an assay time of 20 min, could detect heat-killed Salmonella with a limit of detection of 48 CFU mL-1 , and gave good recoveries in milk. The labels could be stored at 4 °C for 70 days without any deterioration. |
| Databáze: | OpenAIRE |
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