D(-)-lactic acid production by Leuconostoc mesenteroides B512 using different carbon and nitrogen sources
| Autor: | Marcela Piassi Bernardo, Cristian J. Bolner de Lima, Luciana Fontes Coelho, Jonas Contiero |
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| Rok vydání: | 2010 |
| Předmět: |
Cell Extracts
Nitrogen Bioengineering Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Industrial Microbiology Yeasts Molasses Food science Lactic Acid Sugar Molecular Biology Residue (complex analysis) Models Statistical biology Chemistry General Medicine biology.organism_classification Yeast Carbon Lactic acid Culture Media Saccharum Leuconostoc mesenteroides Fermentation Composition (visual arts) Bacteria Algorithms Leuconostoc Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 164(7) |
| ISSN: | 1559-0291 |
| Popis: | Sugar concentration from sugarcane juice and yeast autolysate increased lactic acid production more than the other agro-industrial substrates tested. The concentrations of these two components were further optimized using the Plackett-Burman design and response surface method. A second-order polynomial regression model estimated that a maximal lactic acid production of 66.11 g/L would be obtained when the optimal values of sugar and yeast autolysate were 116.9 and 44.25 g/L, respectively. To validate the optimization of the medium composition, studies were carried out using the optimized conditions to confirm the result of the response surface analysis. After 48 h, lactic acid production using the shake-flask method was at 60.2 g/L. |
| Databáze: | OpenAIRE |
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