Production of somatic cell nuclear transfer embryos using in vitro-grown and in vitro-matured oocytes in rabbits
| Autor: | Yoshihiko Hosoi, Kazuhiro Saeki, Kazuya Matsumoto, Kensaku Kitada, Takeshi Taniguchi, Noriyoshi Oh, Hironobu Sugimoto, Yuta Kida |
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| Rok vydání: | 2014 |
| Předmět: |
Nuclear Transfer Techniques
medicine.drug_class Biology Hydroxamic Acids Animals Genetically Modified Andrology Rate of development medicine Animals Blastocyst Metaphase urogenital system Histone deacetylase inhibitor Embryo Cell Biology Anatomy Embryo Mammalian In vitro In Vitro Oocyte Maturation Techniques In vitro maturation Histone Deacetylase Inhibitors Trichostatin A medicine.anatomical_structure embryonic structures Oocytes Somatic cell nuclear transfer Female Rabbits Developmental Biology medicine.drug |
| Zdroj: | Zygote. 23:494-500 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199414000082 |
| Popis: | SummaryWe examined growing oocytes collected from follicles remaining in superovulated rabbit ovaries, that were grown (in vitro growth, IVG) and matured (in vitro maturation, IVM) in vitro. We produced somatic cell nuclear transfer (SCNT) embryos using the mature oocytes and examined whether these embryos have the ability to develop to the blastocyst stage. In addition, we examined the effects of trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), on the developmental competence of SCNT embryos derived from IVG–IVM oocytes. After growth for 7 days and maturation for 14–16 h in vitro, the growing oocytes reached the metaphase II stage (51.4%). After SCNT, these reconstructed embryos reached the blastocyst stage (20%). Furthermore, the rate of development to the blastocyst stage and the number of cells in the blastocysts in SCNT embryos derived from IVG–IVM oocytes were significantly higher for TSA-treated embryos compared with TSA-untreated embryos (40.6 versus 21.4% and 353.1 ± 59.1 versus 202.5 ± 54.6, P < 0.05). These results indicate that rabbit SCNT embryos using IVG–IVM oocytes have the developmental competence to reach the blastocyst stage. |
| Databáze: | OpenAIRE |
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