In vitro allergic bronchoconstriction in the brown Norway rat
| Autor: | James G. Martin, S Laberge, R J Dandurand, Chong-Gang Wang, David H. Eidelman |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Hypersensitivity
Immediate Male Pulmonary and Respiratory Medicine Serotonin medicine.medical_specialty Pathology Time Factors Ovalbumin Premedication Methysergide Constriction Pathologic In Vitro Techniques Critical Care and Intensive Care Medicine Bronchial Provocation Tests Leukotriene D4 Drug Hypersensitivity Airway resistance Internal medicine medicine Animals Hypersensitivity Delayed Sensitization Bronchus Lung biology business.industry Airway Resistance Immunoglobulin E respiratory system Asthma Bronchodilator Agents Rats Disease Models Animal Endocrinology medicine.anatomical_structure Quinolines biology.protein Bronchoconstriction Propionates medicine.symptom business Airway medicine.drug |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 149:1499-1505 |
| ISSN: | 1535-4970 1073-449X |
| Popis: | The ovalbumin (OA)-sensitized Brown Norway rat (BN) demonstrates early-response (ER) and late-response (LR) allergic bronchoconstriction. To determine whether these responses could be replicated in vitro, we studied lung explants from 8-wk-old male BN rats (wt: 239 +/- 28 g), of which 19 were sensitized to OA (test) and 16 served as controls. Two weeks after sensitization, the animals' lungs were removed, filled with a 1% (wt/vol) agarose-containing solution at 37 degrees C, and cooled to 4 degrees C. Transverse slices (0.5 to 1.0 mm thick) were cut and cultured overnight. Airways were visualized with an inverted microscope and baseline images were obtained with a video camera. To study the ER, 40 airways from 15 test rats and 29 airways from 10 control rats were challenged with 2 micrograms OA and imaged each minute for 10 min. To study the LR, 40 airways from 12 test rats and 44 airways from 12 control rats were challenged with 2 micrograms OA and imaged each hour for 8 h. The maximal response (MR) for each airway was defined as the percent of airway closure. The ER and LR were both defined as an MR > or = mean + 2 SD of the controls. An ER occurred in 38 of 40 test and 2 of 29 control airways (mean MR: 42 +/- 24% versus 4 +/- 3%, p < 0.001), and was completely blocked by methysergide pretreatment in 13 airways.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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