Expression of effector gene SIX1 of Fusarium oxysporum requires living plant cells
| Autor: | Roselinde G.E. Duyvesteijn, Ben J. C. Cornelissen, Martijn Rep, Chris C. N. van Schie, Pieter M. Goltstein, H. Charlotte van der Does, Erik M. M. Manders |
|---|---|
| Přispěvatelé: | Plant Physiology (SILS, FNWI), Cognitive and Systems Neuroscience (SILS, FNWI), Molecular Plant Pathology (SILS, FNWI), Molecular Cytology (SILS, FNWI) |
| Rok vydání: | 2008 |
| Předmět: |
Rhizosphere
Effector Host (biology) Recombinant Fusion Proteins Green Fluorescent Proteins fungi food and beverages Fungus Biology Plant cell biology.organism_classification Plant Roots Microbiology Fungal Proteins Fusarium Solanum lycopersicum Xylem Gene Expression Regulation Fungal Gene expression Fusarium oxysporum Genetics Gene Cells Cultured Plant Diseases |
| Zdroj: | Fungal Genetics and Biology, 45(9), 1257-1264. Academic Press Inc. |
| ISSN: | 1087-1845 |
| DOI: | 10.1016/j.fgb.2008.06.002 |
| Popis: | Fusarium oxysporum is an asexual, soil inhabiting fungus that comprises many different formae speciales, each pathogenic towards a different host plant. In absence of a suitable host all F. oxysporum isolates appear to have a very similar lifestyle, feeding on plant debris and colonizing the rhizosphere of living plants. Upon infection F. oxysporum switches from a saprophytic to an infectious lifestyle, which probably includes the reprogramming of gene expression. In this work we show that the expression of the known effector gene SIX1 of F. oxysporum f. sp. lycopersici is strongly upregulated during colonization of the host plant. Using GFP (green fluorescent protein) as reporter, we show that induction of SIX1 expression starts immediately upon penetration of the root cortex. Induction requires living plant cells, but is not host specific and does not depend on morphological features of roots, since plant cells in culture can also induce SIX1 expression. Taken together, F. oxysporum seems to be able to distinguish between living and dead plant material, preventing unnecessary switches from a saprophytic to an infectious lifestyle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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