Ultraviolet B Radiation Induces a Transient Appearance of IL-4+ Neutrophils, Which Support the Development of Th2 Responses
| Autor: | Sergio Di Nuzzo, Marcel B. M. Teunissen, Regien M. R. Sylva-Steenland, Menno A. de Rie, Gamze Piskin, Jan D. Bos |
|---|---|
| Přispěvatelé: | Dermatology |
| Rok vydání: | 2002 |
| Předmět: |
Adult
Time Factors Neutrophils Ultraviolet Rays medicine.medical_treatment T cell Immunology Lewis X Antigen Tryptase Human skin Biology Blister Th2 Cells Dermis medicine Humans Immunology and Allergy Cells Cultured Interleukin 4 integumentary system Immunomagnetic Separation Papillary dermis Dose-Response Relationship Radiation Molecular biology medicine.anatomical_structure Cytokine Neutrophil Infiltration Cell culture biology.protein Interleukin-4 |
| Zdroj: | Journal of immunology (Baltimore, Md., 168(8), 3732-3739. American Association of Immunologists |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.168.8.3732 |
| Popis: | UVB irradiation can cause considerable changes in the composition of cells in the skin and in cutaneous cytokine levels. We found that a single exposure of normal human skin to UVB induced an infiltration of numerous IL-4+ cells. This recruitment was detectable in the papillary dermis already 5 h after irradiation, reaching a peak at 24 h and declining gradually thereafter. The IL-4+ cells appeared in the epidermis at 24 h postradiation and reached a plateau at days 2 and 3. The number of IL-4+ cells was markedly decreased in both dermis and epidermis at day 4, and at later time points, the IL-4 expression was absent. The IL-4+ cells did not coexpress CD3 (T cells), tryptase (mast cells), CD56 (NK cells), and CD36 (macrophages). They did coexpress CD15 and CD11b, showed a clear association with elastase, and had a multilobed nucleus, indicating that UVB-induced infiltrating IL-4+ cells are neutrophils. Blister fluid from irradiated skin, but not from control skin, contained IL-4 protein as well as increased levels of IL-6, IL-8, and TNF-α. In contrast to control cultures derived from nonirradiated skin, a predominant type 2 T cell response was detected in T cells present in primary dermal cell cultures derived from UVB-exposed skin. This type 2 shift was abolished when CD15+ cells (i.e., neutrophils) were depleted from the dermal cell suspension before culturing, suggesting that neutrophils favor type 2 T cell responses in UVB-exposed skin. |
| Databáze: | OpenAIRE |
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