Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia
| Autor: | Kyu Hyeon Kim, Jae Hun Jeong, Hyeong Seok An, Eun Bee Choi, Yu Jeong Ahn, Jong Youl Lee, Kyung Eun Kim, Jaewoong Lee, Hyun Joo Shin, Gu Seob Roh, Eun Ae Jeong, Hye Min Jang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Physiology Clinical Biochemistry Ferroportin Transferrin receptor ob/ob mouse RM1-950 medicine.disease_cause Biochemistry Article sarcopenia 03 medical and health sciences 0302 clinical medicine iron Hepcidin Internal medicine medicine oxidative stress Molecular Biology biology Chemistry lipocalin-2 Skeletal muscle Cell Biology medicine.disease Ferritin 030104 developmental biology Endocrinology medicine.anatomical_structure inflammation Sarcopenia biology.protein Therapeutics. Pharmacology 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Antioxidants, Vol 10, Iss 758, p 758 (2021) Antioxidants Volume 10 Issue 5 |
| ISSN: | 2076-3921 |
| Popis: | Obesity and insulin resistance accelerate aging-related sarcopenia, which is associated with iron load and oxidative stress. Lipocalin-2 (LCN2) is an iron-binding protein that has been associated with skeletal muscle regeneration, but details regarding its role in obese sarcopenia remain unclear. Here, we report that elevated LCN2 levels in skeletal muscle are linked to muscle atrophy-related inflammation and oxidative stress in leptin-deficient ob/ob mice. RNA sequencing analyses indicated the LCN2 gene expression is enhanced in skeletal muscle of ob/ob mice with sarcopenia. In addition to muscular iron accumulation in ob/ob mice, expressions of iron homeostasis-related divalent metal transporter 1, ferritin, and hepcidin proteins were increased in ob/ob mice compared to lean littermates, whereas expressions of transferrin receptor and ferroportin were reduced. Collectively, these findings demonstrate that LCN2 functions as a potent proinflammatory factor in skeletal muscle in response to obesity-related sarcopenia and is thus a therapeutic candidate target for sarcopenia treatment. |
| Databáze: | OpenAIRE |
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