USF Inhibits Cell Proliferation through Delay in G2/M Phase in FRTL-5 Cells
| Autor: | Yong Ki Min, Moon-Kyu Lee, Yun Jae Chung, Hye Seung Jung, Kwang-Won Kim, Jae Hoon Chung, Hyun-Kyung Chung, Myung-Shik Lee, Keun-Sook Kim |
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| Rok vydání: | 2007 |
| Předmět: |
G2 Phase
endocrine system Cell type Endocrinology Diabetes and Metabolism Thyroid Gland Thyrotropin Apoptosis Cyclin B Biology Transfection Upstream Stimulatory Factor Endocrinology Cyclin-dependent kinase CDC2 Protein Kinase Cyclic AMP Animals Thyroid Neoplasms Cyclin B1 Cells Cultured Cell Proliferation Cell growth Cell cycle Molecular biology Carcinoma Papillary Bromodeoxyuridine Cell culture biology.protein Upstream Stimulatory Factors Cell Division Cyclin-Dependent Kinase Inhibitor p27 Thymidine |
| Zdroj: | Endocrine Journal. 54:275-285 |
| ISSN: | 1348-4540 0918-8959 |
| DOI: | 10.1507/endocrj.k06-120 |
| Popis: | Upstream stimulatory factor (USF) has a negative effect on the cell proliferation in some cell types. However, its effect on thyrocytes is not clear. Therefore, we investigated the effects of USF on the proliferation and function of thyroid follicular cells. Complementary DNAs of the USF-1 and USF-2 were synthesized using RT-PCR from FRTL-5 cells, and each was transfected to FRTL-5 cells and papillary thyroid carcinoma cell lines. Cyclic AMP (cAMP) production and [methyl-3H] thymidine uptake after thyroid stimulating hormone (TSH) treatment were measured in FRTL-5 cells. In the carcinoma cell lines, 5-bromo-2'-deoxyuridine (BrdU) uptake was assayed to evaluate cell proliferation. Apoptosis was tested by Hoechst staining and cell cycle analysis was done using a fluorescence activated cell sorting. Expression of cell cycle regulating genes was evaluated by Northern and Western blotting. Overexpression of USF-1 and USF-2 significantly suppressed TSH-stimulated [methyl-3H] thymidine uptake (p |
| Databáze: | OpenAIRE |
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