Infant acute lymphoblastic leukemia – combined cytogenetic, immunophenotypical and molecular analysis of 77 cases
| Autor: | S. Pils, Gritta Janka-Schaub, Martin Zimmermann, Susanne Viehmann, Martin Stanulla, Jochen Harbott, Christian Wuchter, Martin Schrappe, Andrea Teigler-Schlegel, Wolf-Dieter Ludwig, Arndt Borkhardt |
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| Rok vydání: | 2002 |
| Předmět: |
Cancer Research
medicine.medical_specialty Biology Sensitivity and Specificity Disease-Free Survival Immunophenotyping Antigens CD Bone Marrow hemic and lymphatic diseases Acute lymphocytic leukemia medicine Humans Prospective Studies neoplasms In Situ Hybridization Fluorescence Southern blot Chromosome Aberrations Gene Rearrangement medicine.diagnostic_test Chromosomes Human Pair 11 Infant Newborn Cytogenetics Infant Karyotype Hematology Gene rearrangement Precursor Cell Lymphoblastic Leukemia-Lymphoma Prognosis medicine.disease Molecular biology Infant Acute Lymphoblastic Leukemia Blotting Southern Treatment Outcome Oncology Karyotyping Fluorescence in situ hybridization |
| Zdroj: | Leukemia. 16:1685-1690 |
| ISSN: | 1476-5551 0887-6924 |
| Popis: | We used karyotyping, fluorescence in situ hybridization (FISH), Southern blotting, and RT-PCR in order to analyze prospectively 77 infants (less than 1 year of age) with acute lymphoblastic leukemia for the occurrence of 11q23/MLL rearrangements and/or other cytogenetic abnormalities. Out of the 69 informative samples we found an 11q23/MLL rearrangement in 42 cases (61%). Regarding only pro-B ALL cases, the incidence of 11q23/MLL rearranged cases, however, reached more than 90% The infants were treated within the therapy studies ALL-BFM90, ALL-BFM95 and CoALL-05-92. For patients with an adequate follow-up of 4 years the event-free survival of the 11q23/MLL-positive and 11q23/MLL-negative group was 0.2 or 0.64, respectively (P = 0.024). The monoclonal antibody 7.1. (moab 7.1) does not react with normal hematopoetic precursors or mature blood cells but was shown to specifically react with leukemic cells bearing a rearrangement of chromosome 11q23 or the MLL gene, respectively. We, therefore, specifically addressed the question whether the reactivity of moab 7.1, as determined by flow cytometry, may substitute for molecular testing of an 11q23/MLL rearrangement in this cohort of infant ALLs. Reactivity of moab 7.1 indicated a 11q23/MLL rearrangement with a specificity of 100%. However, five of the 11q23/MLL-positive cases did not react with moab 7.1 indicating a sensitivity of 84% only. Three of these five moab 7.1-negative but 11q23/MLL-positive cases could be identified by their unique expression pattern of CD65s and/or CD15. Thus, 95% of all 11q23/MLL-positive ALL cases in infancy may be identified by flow cytometry based on their expression of CD15, CD65s and/or moab 7.1. |
| Databáze: | OpenAIRE |
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