In Vivo Response of Acellular Porcine Pericardial for Tissue Engineered Transcatheter Aortic Valves
| Autor: | Amir Lerman, Jason L. Go, Christopher Noble, Melissa D. Young, David Morse, Reza Khorramirouz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Aortic valve CD31 Pathology medicine.medical_specialty Swine lcsh:Medicine Article Extracellular matrix Rats Sprague-Dawley Transcatheter Aortic Valve Replacement 03 medical and health sciences 0302 clinical medicine Tissue engineering In vivo medicine Pericardium Animals Heart valve lcsh:Science Bioprosthesis Multidisciplinary Tissue Engineering business.industry lcsh:R Mesenchymal stem cell Mesenchymal Stem Cells Rats 030104 developmental biology medicine.anatomical_structure Aortic Valve Heart Valve Prosthesis cardiovascular system lcsh:Q business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Current heart valve prostheses have limitations that include durability, inability to grow in pediatric patients, and lifelong anticoagulation. Transcatheter aortic valve replacements are minimally invasive procedures, and therefore have emerged as an alternative to traditional valve prostheses. In this experiment, the regenerative capacity of potential tissue engineered transcatheter valve scaffolds (1) acellular porcine pericardium and (2) mesenchymal stem cell-seeded acellular porcine pericardium were compared to native porcine aortic valve cusps in a rat subcutaneous model for up to 8 weeks. Immunohistochemistry, extracellular matrix evaluation, and tissue biomechanics were evaluated on the explanted tissue. Acellular valve scaffolds expressed CD163, CD31, alpha smooth muscle actin, and vimentin at each time point indicating host cell recellularization; however, MSC-seeded tissue showed greater recellularization. Inflammatory cells were observed with CD3 biomarker in native porcine pericardial tissue throughout the study. No inflammation was observed in either acellular or MSC-seeded scaffolds. There was no mechanical advantage observed in MSC-seeded tissue; however after the first week post-explant, there was a decrease in mechanical properties in all groups (p |
| Databáze: | OpenAIRE |
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