Reduced Cerebral Glucose Uptake in an Alzheimer’s Rat Model With Glucose-Weighted Chemical Exchange Saturation Transfer Imaging
| Autor: | Qianqian Wang, Zhuozhi Dai, Bingna Zhang, Yuanyu Shen, Zhiwei Shen, Renhua Wu, Peidong Chen, Zerui Zhuang, Yanzhi Chen, Jiefen Lin |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Aging medicine.medical_specialty glucose metabolism Cognitive Neuroscience Glucose uptake Morris water navigation task Hippocampus Carbohydrate metabolism lcsh:RC321-571 03 medical and health sciences symbols.namesake chemistry.chemical_compound 0302 clinical medicine In vivo D-Glucose Internal medicine medicine magnetic resonance imaging lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research medicine.diagnostic_test Chemistry glucoCEST Magnetic resonance imaging 030104 developmental biology Endocrinology Nissl body symbols Alzheimer’s disease D-glucose 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Aging Neuroscience Frontiers in Aging Neuroscience, Vol 13 (2021) |
| ISSN: | 1663-4365 |
| Popis: | A correlation between the abnormal cerebral glucose metabolism and the progression of Alzheimer’s disease (AD) has been found in previous studies, suggesting that glucose alterations may be used to predict the histopathological diagnosis in AD. In this study, we investigated the dynamic changes of cerebral glucose uptake in vivo using MR glucose chemical exchange saturation transfer (glucoCEST) imaging in a rat model of AD with an intracerebroventricular (i.c.v) injection of amyloid Aβ-protein (25–35), confirmed by Morris water maze and Nissl staining. In total, 6 rats in the AD group and 6 rats in the control group that were given an injection of sterile normal saline were included. At 28 days after injection, all rats performed a 7.0 T MR exanimation, including glucoCEST, diffusion tensor imaging (DTI) and hippocampus magnetic resonance spectra (MRS), to detect the possible metabolic and structural changes in the rat brain. A significantly elevated brain glucoCEST signal in the brain of AD rats was observed, and a decreased brain glucose uptake was also explored during the progression of glucose infusion compared with those in rats of the control group. In addition, there is a significant positive correlation between glucoCEST enhancement (GCE) and myo-Inosito (Ins) in the AD group and the control group (P < 0.05). A significantly reduced number of neurons in the cortex and hippocampus in AD rats combined with the significantly longer escape and a decreased number of crossings were verified at 28 days after Aβ25–35 injection by Nissl staining and Morris water maze, respectively. Our results indicated that an abnormal brain glucose mechanism in AD rats could be detected by glucoCEST imaging, suggesting a new method to explore the occurrence and progress of diabetes-related AD or dementia. |
| Databáze: | OpenAIRE |
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