Efficacy of the amphibian peptide distinctin in a neutropenic mouse model of staphylococcal sepsis
| Autor: | Fiorenza Orlando, Anna Maria Salzano, Stefania De Luca, Carmela Silvestri, Vittorio Saba, Oscar Cirioni, Federico Mocchegiani, Andrea Giacometti, Giorgio Scalise, Roberto Ghiselli, Andrea Scaloni |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Staphylococcus aureus Neutropenia Peptide Microbial Sensitivity Tests Critical Care and Intensive Care Medicine medicine.disease_cause Staphylococcal infections Hemolysis Amphibian Proteins Sepsis Mice Random Allocation Pharmacotherapy Intensive care Animals Medicine chemistry.chemical_classification Mice Inbred BALB C Leukopenia Dose-Response Relationship Drug business.industry Staphylococcal Infections medicine.disease Anti-Bacterial Agents Disease Models Animal chemistry Immunology Drug Therapy Combination Anura medicine.symptom business Antimicrobial Cationic Peptides |
| Zdroj: | Critical Care Medicine. 36:2629-2633 |
| ISSN: | 0090-3493 |
| Popis: | To investigate the efficacy of distinctin in a neutropenic mouse model of staphylococcal sepsis.Prospective, randomized, and controlled animal study.Research laboratory in a University Hospital.BALB/c male mice.Mice were rendered neutropenic by injecting cyclophosphamide (200 mg/kg of body weight/day) on days -4 and -2 preinfection. Infection was induced at time 0 by intraperitoneal injection of 1 x 10(9) colony forming units of the staphylococcal strain. For each model, all animals were randomized to receive intravenous isotonic sodium chloride solution, 1 mg/kg distinctin, and 10 mg/kg imipenem, 10 mg/kg vancomycin, 10 mg/kg teicoplanin or 10 mg/kg linezolid alone, or combined with 1 mg/kg distinctin.Lethality, bacterial growth in blood and peritoneum, spleen, liver, and mesenteric lymph nodes.All combined regimen showed lower lethality rates than singly treated-groups. Distinctin plus vancomycin or teicoplanin exerted the lowest lethality rate. All regimens were significantly superior to controls at reducing blood, spleen, peritoneum, liver and mesenteric lymph node complex bacterial burdens, whereas all combined treated groups were higher effective than singly treated groups.Our data indicate that distinctin alone or combined with other antibiotics may be useful in treating severe staphylococcal infections. |
| Databáze: | OpenAIRE |
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