Elucidation of tellurium biogenic nanoparticles in garlic, Allium sativum, by inductively coupled plasma-mass spectrometry
| Autor: | Keita Kobayashi, Yu-ki Tanaka, Yasumitsu Ogra, Akiko Hokura, Shohei Takada, Kazuhiro Kumagai |
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| Rok vydání: | 2020 |
| Předmět: |
Scanning electron microscope
Nanoparticle chemistry.chemical_element 010501 environmental sciences Mass spectrometry Plant Roots 01 natural sciences Biochemistry Mass Spectrometry Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Garlic Inductively coupled plasma mass spectrometry 0105 earth and related environmental sciences food and beverages Allium sativum chemistry Elemental analysis Transmission electron microscopy Nanoparticles Molecular Medicine Tellurium 030217 neurology & neurosurgery Nuclear chemistry |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 62:126628 |
| ISSN: | 0946-672X |
| DOI: | 10.1016/j.jtemb.2020.126628 |
| Popis: | Background Biosynthesis of Te nanoparticles may occur in higher plants exposed to Te, as reported on microorganisms. However, unambiguous observations of the biogenic nanoparticles (BgNPs) of Te in plants are lacking. Hence, in this study, we investigated the formation of insoluble BgNPs of Te in garlic (Allium sativum) as a model plant. Method We performed elemental analysis based on inductively coupled plasma-mass spectrometry (ICP-MS) technique, and obtained Te concentration and distribution in various parts of garlic. In addition, insoluble Te particles were detected by fast time-resolved ICP-MS. Direct observation of the insoluble Te particle was also conducted by scanning electron microscope (SEM) and transmission electron microscope (TEM). Results A part of the roots and clove from Te-exposed garlic showed black coloration. Te concentrations in the black-colored parts were significantly increased compared with the non-colored parts. Transient signals of Te unique to nanoparticles were detected from the insoluble fractions of the black-colored parts. Finally, rod-shaped biogenic Te nanoparticles consisting of highly crystalline elemental Te was observed by SEM and TEM. Conclusion Our data provide new insights to the metabolic pathway of Te in higher plants for the formation of insoluble biogenic nanoparticles, which is extremely important for the detoxification of Te. |
| Databáze: | OpenAIRE |
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