IL6 Receptor Facilitates Adipogenesis Differentiation of Human Mesenchymal Stem Cells through Activating P38 Pathway
| Autor: | Yanfeng Wu, Huiyong Shen, Huadi Chen, Hongjun Su, Zhongyu Xie, Wen Deng, Xiaohua Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
MAPK/ERK pathway
0303 health sciences Gene knockdown Chemistry Cellular differentiation p38 mitogen-activated protein kinases Mesenchymal stem cell Mesenchymal stem cells (MSCs) P38 Adipogenesis differentiation Cell Biology Interleukin 6 (IL6) Cell biology Blot 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adipogenesis Interleukin 6 receptor (IL6R) Oil Red O Original Article 030217 neurology & neurosurgery 030304 developmental biology Developmental Biology |
| Zdroj: | International Journal of Stem Cells |
| ISSN: | 2005-5447 2005-3606 |
| Popis: | Background and Objectives Mesenchymal stem cells (MSCs) have the multipotent capacity to differentiate into multiple tissue lineages as well as to self-renew, which is the main origin of adipocytes. IL6/IL6R pathway exerts a significant role in tissue regeneration and cell differentiation. Whereas, the underlying mechanism between IL6/IL6R pathway and MSCs adipogenesis differentiation remains elusive. Methods MSCs from healthy donors were cultured in adipogenesis differentiation medium for 0∼14 days, during which their adipogenesis differentiation degree was evaluated by Oil Red O staining. The expression of IL6R was detected in MSCs during adipogenesis differentiation. Knockdown and overexpression of IL6R were respectively performed using siRNA and lentivirus to investigate its effect on MSCs adipogenesis differentiation. The adipogenesis marker genes expression and MAPK pathway activation were detected by Western blotting. The role of P38 pathway in the adipogenesis differentiation of MSCs was determined using the specific inhibitor SB203580. Results The expression of IL6 and IL6R increased during adipogenesis differentiation in MSCs, which were positively correlated with Oil Red O quantification result. Knockdown and overexpression experiments demonstrated a positive correlation between the expressions of IL6R and MSCs adipogenesis differentiation, accompanied by same trend of P38 phosphorylation. Besides, the specific P38 inhibitor SB203580 markedly inhibited the adipogenesis differentiation potential of MSCs. Conclusions This study reveals IL6R facilitates the adiogenesis differentiation of MSCs via activating P38 pathway. |
| Databáze: | OpenAIRE |
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