Jasmonate Precursor Biosynthetic Enzymes LOX3 and LOX4 Control Wound-Response Growth Restriction
| Autor: | Edward E. Farmer, Stéphanie Stolz, Tsu-Hao Yang, Gaétan Glauser, Aurore Lenglet-Hilfiker |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Genotype Physiology Mutant Lipoxygenase Arabidopsis Plant Science Cyclopentanes Spodoptera medicine.disease_cause Genes Plant 01 natural sciences Gene Expression Regulation Plant Genetics medicine Arabidopsis thaliana Animals Jasmonate Oxylipins Gene News and Views Research Articles Regulation of gene expression Mutation Enzyme Precursors biology Arabidopsis Proteins fungi Genetic Variation food and beverages Lipoxygenases biology.organism_classification Cell biology Phenotype Phloem 010606 plant biology & botany |
| Zdroj: | Plant Physiol Plant physiology, vol. 184, no. 2, pp. 1172-1180 |
| Popis: | Wound-response plant growth restriction requires the synthesis of potent mediators called jasmonates (JAs). Four 13-lipoxygenases (13-LOXs) produce JA precursors in Arabidopsis (Arabidopsis thaliana) leaves, but the 13-LOXs responsible for growth restriction have not yet been identified. Through loss-of-function genetic analyses, we identified LOX3 and LOX4 as the principal 13-LOXs responsible for vegetative growth restriction after repetitive wounding. Additional genetic studies were carried out in the gain-of-function fatty acid oxygenation 2 (fou2) mutant that, even when undamaged, shows JA-dependent leaf growth restriction. The fou2 lox3 lox4 triple mutant suppressed the fou2 JA-dependent growth phenotype, confirming that LOX3 and LOX4 function in leaf growth restriction. The fou2 mutation affects the TWO PORE CHANNEL1 (TPC1) ion channel. Additional genetic approaches based on this gene were used to further investigate LOX3 function in relation to leaf growth. To activate LOX3-dependent JA production in unwounded plants, we employed hyperactive TPC1 variants. Expression of the TPC1ΔCa i variant in phloem companion cells caused strongly reduced rosette growth in the absence of wounding. Summarizing, in parallel to their established roles in male reproductive development in Arabidopsis, LOX3 and LOX4 control leaf growth rates after wounding. The process of wound-response growth restriction can be recapitulated in unwounded plants when the LOX3 pathway is activated genetically using a hyperactive vacuolar cation channel. |
| Databáze: | OpenAIRE |
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