Induction of CYP1A mRNA in Carp (Cyprinus carpio) from the Kalamazoo River polychlorinated biphenyl-contaminated superfund site and in a laboratory study
| Autor: | Charles F. Ide, Jay C. Means, Marla A. Fisher, C. Mehne |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Michigan
Carps Health Toxicology and Mutagenesis Toxicology medicine.disease_cause Microbiology Cyprinus Superoxide dismutase chemistry.chemical_compound Rivers medicine Cytochrome P-450 CYP1A1 Ecotoxicology Animals RNA Messenger Carp biology Ecology Reverse Transcriptase Polymerase Chain Reaction Cytochrome P450 Polychlorinated biphenyl Gene Expression Regulation Developmental Proteins General Medicine biology.organism_classification Pollution Polychlorinated Biphenyls Oxidative Stress chemistry Liver Enzyme Induction biology.protein sense organs Phosphoenolpyruvate carboxykinase Laboratories Oxidative stress |
| Zdroj: | Archives of environmental contamination and toxicology. 50(1) |
| ISSN: | 0090-4341 |
| Popis: | The Kalamazoo River Superfund site in Michigan is contaminated with polychlorinated biphenyls (PCBs), which were heavily discharged into the river from several paper companies as part of the deinking process in the 1950s through 1970s. We characterized biomarkers of chronic PCB exposure in a resident fish population using real-time reverse transcriptase–polymerase chain reaction to examine mRNA expression levels of multiple genes in carp (Cyprinus carpio) liver from PCB contaminated and reference sites in the Kalamazoo River. We also measured these same genes in juvenile carp exposed to dietary PCBs for 4 months. Kalamazoo River carp had significantly increased levels of cytochrome P450 1A (CYP1A) mRNA as did carp fed PCBs in the laboratory. No significant mRNA upregulation occurred in the specific oxidative stress genes (gamma-glutamylcysteine synthetase and magnesium superoxide dismutase) and metabolic genes (phosphoenolpyruvate carboxykinase and nucleolin) examined. These data are consistent with the idea that carp from the Kalamazoo River Superfund Site are responding to PCB exposure via upregulation of CYP1A independent of activation of the oxidative stress response genes normally thought to be co-regulated with CYP1A. |
| Databáze: | OpenAIRE |
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