Proteomic analysis of Metarhizium anisopliae secretion in the presence of the insect pest Callosobruchus maculatus
| Autor: | Ângela Mehta, Eliane Ferreira Noronha, Caroline D. Pereira, Fabio T. M. Costa, Ruy de Araujo Caldas, André M. Murad, Robert N.G. Miller, Octavio L. Franco |
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| Rok vydání: | 2008 |
| Předmět: |
Proteomics
Metarhizium Insecta media_common.quotation_subject Metarhizium anisopliae Insect Genetically modified crops Microbiology Mass Spectrometry Fungal Proteins Vigna Crop Botany Animals Electrophoresis Gel Two-Dimensional Pest Control Biological media_common biology business.industry fungi Pest control food and beverages Pesticide biology.organism_classification Coleoptera Callosobruchus maculatus Agronomy business |
| Zdroj: | Microbiology. 154:3766-3774 |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | Crop improvement in agriculture generally focuses on yield, seed quality and nutritional characteristics, as opposed to resistance to biotic stresses. Consequently, natural antifeedant toxins are often rare in seed material, with commercial crops being prone to insect pest predation. In the specific case of cowpea (Vigna unguiculata), smallholder cropping is affected by insect pests that reproduce inside the stored seeds. Entomopathogenic organisms can offer an alternative to conventional pesticides for pest control, producing hydrolases that degrade insect exoskeleton. In this study, protein secretions of the ascomycete Metarhizium anisopliae, which conferred bioinsecticidal activity against Callosobruchus maculatus, were characterized via 2D electrophoresis and mass spectrometry. Proteases, reductases and acetyltransferase enzymes were detected. These may be involved in degradation and nutrient uptake from dehydrated C. maculatus. Proteins identified in this work allowed description of metabolic pathways. Their potential applications in biotechnology include both novel compound development and production of genetically modified plants resistant to insect pests. |
| Databáze: | OpenAIRE |
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