New Metabolites From the Co-culture of Marine-Derived Actinomycete Streptomyces rochei MB037 and Fungus Rhinocladiella similis 35
| Autor: | Meilin Yu, Yingxin Li, Shivakumar P. Banakar, Lu Liu, Changlun Shao, Zhiyong Li, Changyun Wang |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Nitrile Stereochemistry lcsh:QR1-502 Fungus medicine.disease_cause Microbiology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound antibacterial activity medicine 030304 developmental biology 0303 health sciences Natural product biology 030306 microbiology fungus biology.organism_classification co-culture borrelidin Microbial population biology chemistry Staphylococcus aureus Chromone actinomycete Antibacterial activity Derivative (chemistry) |
| Zdroj: | Frontiers in Microbiology, Vol 10 (2019) |
| ISSN: | 1664-302X |
| Popis: | Co-culture of different microbes simulating the natural state of microbial community may produce potentially new compounds because of nutrition or space competition. To mine its metabolic potential in depth, co-culture of Streptomyces rochei MB037 with a gorgonian-derived fungus Rhinocladiella similis 35 was carried out to stimulate the production of new metabolites in this study, using pure cultivation as control. Five metabolites were isolated successfully from co-culture broth, including two new fatty acids with rare nitrile group, borrelidins J and K (1 and 2), one chromone derivative as a new natural product, 7-methoxy-2,3-dimethylchromone-4-one (3), together with two known 18-membered macrolides, borrelidin (4) and borrelidin F (5). The structures of 1–3 were elucidated by using a combination of NMR and MS spectroscopy, ester hydrolysis, and optical rotation methods. Interestingly, 1 and 2 were obtained only in co-culture. Though 3 was gained from either co-culture or single culture, its production was increased significantly by co-culture. Compound 1 exhibited significant antibacterial activity against methicillin-resistant Staphylococcus aureus with a MIC value of 0.195 μg/mL. |
| Databáze: | OpenAIRE |
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