Killing of Treponema denticola by Mouse Peritoneal Macrophages
| Autor: | Luisa Miragliotta, Jacques Izard, Paolo Gaibani, Vittorio Sambri, Caterina Vocale, Maria Teresa Pellegrino, Francesca Cavrini, Simone Ambretti |
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| Přispěvatelé: | Gaibani P, Vocale C, Ambretti S, Cavrini F, Izard J, Miragliotta L, Pellegrino MT, Sambri V. |
| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Phagocytosis Motility Biology medicine.disease_cause PHAGOCYTOSIS Microbiology Mice Immune system Bacterial Proteins stomatognathic system IMMUNE RESPONSE Escherichia coli medicine Animals Anaerobiosis MACROPHAGES Fluorescent Antibody Technique Indirect General Dentistry Cells Cultured Innate immune system TREPONEMA DENTICOLA Tumor Necrosis Factor-alpha Membrane Proteins Research Reports Treponema denticola Immunoglobulin E biology.organism_classification Antibodies Bacterial Aerobiosis Antibody opsonization Cytoskeletal Proteins stomatognathic diseases Mutation Macrophages Peritoneal Tumor necrosis factor alpha PERIODONTITIS |
| Zdroj: | Journal of Dental Research. 89:521-526 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | Treponema denticola has been identified as an important cause of periodontal disease and hypothesized to be involved in extra-oral infections. The objective of this study was to investigate the role of T. denticola cell length and motility during mouse peritoneal macrophages in vitro uptake. Macrophages, incubated under aerobic and anaerobic conditions, produced a similar amount of TNF-α when stimulated with Escherichia coli LPS. The uptake of FlgE- and CfpA-deficient mutants of T. denticola was significantly increased compared with the wild-type strain, due to cell size or lack of motility. Opsonization with specific antibodies considerably improved the treponemes’ uptake. These results suggest that macrophages, in addition to other phagocytes, could play an important role in the control of T. denticola infection, and that the raising of specific antibodies could improve the efficacy of the immune response toward T. denticola, either at an oral site or during dissemination. |
| Databáze: | OpenAIRE |
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