Trigonella foenum-graecum L. Sprouted Seed Extract: Its Chemical HPLC Analysis, Abortive Effect, and Neurodevelopmental Toxicity on Mice
| Autor: | Rachida Aboufatima, Jawad Laadraoui, Zahra Sokar, Abderrahman Chait, Sara Oufquir, Zineb El Gabbas, Mehdi Ait Laaradia, Kenza Bezza |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Pregnancy Trigonella Antioxidant Traditional medicine Article Subject Offspring medicine.medical_treatment Developmental toxicity Biology medicine.disease biology.organism_classification 01 natural sciences 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences Other systems of medicine Complementary and alternative medicine Toxicity medicine Reproductive toxicity RZ201-999 030304 developmental biology Sprouting Research Article |
| Zdroj: | Evidence-based Complementary and Alternative Medicine : eCAM Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020) |
| ISSN: | 1741-427X |
| DOI: | 10.1155/2020/1615794 |
| Popis: | The Trigonella foenum-graecum L. seeds, in a dormant or sprouted state, have been largely investigated for their therapeutic activities such as being antidiabetic, antioxidant, cholesterol-lowering, and as a digestive enhancer too. Nevertheless, there are no studies evaluating the potential developmental toxicity of germinated grains despite the availability of numerous research studies demonstrating the teratogenicity effect of unsprouted seeds. Therefore, this research work was conducted to assess the impact of fenugreek sprouts on maternal and neurobehavioral developmental toxicities on mice. The lyophilized aqueous extract of germinated seeds was administered via oral gavage on a daily basis to five groups of mated female mice throughout pregnancy at doses of 200, 500, 800, and 1000 mg/kg/day and the control group was administered distilled water. Maternal reproductive toxicity was evaluated, and the surviving pups were assessed for their physical development, malformation, and neurobehavioral toxicity by using a battery of tests from birth to the 25th postnatal day. Additionally, the aqueous extract of germinated and ungerminated seeds was analyzed by high-performance liquid chromatography (HPLC) for a comparison of their major compounds. For pregnant treated female mice, no death and no intoxication symptoms have been registered during the test. However, the sprouts’ extract has provoked a significant decrease in fertility, spontaneous abortion, pup’s mortality, and neurobehavioral disorder in offspring. HPLC analysis reveals an increase in total phenolic compound concentration by the process of sprouting. |
| Databáze: | OpenAIRE |
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