Oncogenic comparison of human papillomavirus type 58 E7 variants
| Autor: | Grace Py Cheung, Chenghua Hu, Raymond Wm Lung, Miranda Thomas, David Pim, Paul K.S. Chan, Priscilla T. Y. Law, Siaw Shi Boon, Wendy C. S. Ho, Lawrence Banks, Zigui Chen, Paola Massimi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
HPV58 Carcinogenesis Papillomavirus E7 Proteins Uterine Cervical Neoplasms Oncogenicity Biology medicine.disease_cause 3T3 cells 03 medical and health sciences 0302 clinical medicine oncogenicity Cell Line Tumor medicine Humans Human papillomavirus human papillomavirus Papillomaviridae Cervical cancer chemistry.chemical_classification Effector Papillomavirus Infections T20I/G63S Cell Biology Original Articles medicine.disease Phenotype Amino acid 030104 developmental biology medicine.anatomical_structure chemistry variant 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female Original Article HeLa Cells |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 |
| Popis: | Human papillomavirus 58 (HPV58) ranks the second or third in East Asian cervical cancers. Current studies on HPV58 are scarce and focus on the prototype. Previously, we identified the three most common circulating HPV58 E7 strains contained amino acid alterations: G41R/G63D (51%), T20I/G63S (22%) and T74A/D76E (14%) respectively. Among them, the T20I/G63S variant (V1) had a stronger epidemiological association with cervical cancer. We therefore suggested that V1 possessed stronger oncogenicity than the other two variants. Here, we performed phenotypic assays to characterize and compare their oncogenicities with HPV58 E7 prototype. Our results showed that overexpression of V1 conferred a higher colony‐forming ability to primary murine epithelial cells than prototype (P |
| Databáze: | OpenAIRE |
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