Amino Acid Specific Effects on RNA Tertiary Interactions: Single-Molecule Kinetic and Thermodynamic Studies
| Autor: | David J. Nesbitt, Hsuan-Lei Sung, Abhigyan Sengupta |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Arginine Stereochemistry Lysine RNA 010402 general chemistry 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Amino acid 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Betaine chemistry Materials Chemistry Side chain Amine gas treating Proline Physical and Theoretical Chemistry |
| Zdroj: | The Journal of Physical Chemistry B. 120:10615-10627 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/acs.jpcb.6b05840 |
| Popis: | In light of the current models for an early RNA-based universe, the potential influence of simple amino acids on tertiary folding of ribozymal RNA into biochemically competent structures is speculated to be of significant evolutionary importance. In the present work, the folding–unfolding kinetics of a ubiquitous tertiary interaction motif, the GAAA tetraloop–tetraloop receptor (TL–TLR), is investigated by single-molecule fluorescence resonance energy transfer spectroscopy in the presence of natural amino acids both with (e.g., lysine, arginine) and without (e.g., glycine) protonated side chain residues. By way of control, we also investigate the effects of a special amino acid (e.g., proline) and amino acid mimetic (e.g., betaine) that contain secondary or quaternary amine groups rather than a primary amine group. This combination permits systematic study of amino acid induced (or amino acid like) RNA folding dynamics as a function of side chain complexity, pKa, charge state, and amine group content. Mos... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|