Collagen synthesis in cells cultured from v. Recklinghausen's neurofibromatosis
| Autor: | Risto Penttinen, Timo Vihersaari, S. Renvall, Juha Peltonen, T. Marttala |
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| Rok vydání: | 1981 |
| Předmět: |
Pathology
medicine.medical_specialty Neurofibromatosis 1 business.industry Middle Aged medicine.disease Median Nerve Pathology and Forensic Medicine Cellular and Molecular Neuroscience Peripheral Nervous System Neoplasms medicine Humans Female Collagen Neurology (clinical) Neurofibromatosis Recklinghausen's neurofibromatosis business Cells Cultured Total protein |
| Zdroj: | Acta Neuropathologica. 55:183-187 |
| ISSN: | 1432-0533 0001-6322 |
| DOI: | 10.1007/bf00691316 |
| Popis: | Subcutaneous tumors of a patient with v. Recklinghausen's neurofibromatosis contained about 31% collagen calculated on the basis of lipid-free dry weight. Slices of the tumors synthesized collagen at a rate (4.7-8.5% from total protein) which was higher than that of the skin slices (2.8-5.9%). Neurofibromatosis cells were cultured from tumors of two patients. They synthesized relatively much more collagen than cultures of skin fibroblasts of the same patient or of healthy age-matched control persons. The second patient's cultures were studied in detail. The cell densities of these cultures were higher and expressed more variation than the densities of control skin fibroblasts. Ion exchange cellulose chromatograms, SDS-polyacrylamide gel electrophoresis and 3-hydroxyproline analysis of the radioactive proteins made by the cultures indicate that most of the collagenous proteins resembled type I collagen. High proliferative capacity and high collagen synthesis of selected neurofibromatosis cells explains the growth of solid tumors. |
| Databáze: | OpenAIRE |
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